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| 普通小麦CONSTANS-LIKE(COL)基因家族的鉴定和表达模式分析 |
| Identification and Expression Pattern Analysis of CONSTANS-LIKE (COL) Gene Family in Bread Wheat (Triticum aestivum L.) |
| 投稿时间：2024-02-01 修订日期：2024-04-18 |
| DOI： |
| 中文关键词: 小麦 COL基因家族 表达模式 结构特点 |
| 英文关键词:Wheat COL gene family expression pattern structural characteristics |
| 基金项目:陕西省重点研发项目 （编号：2021ZDLNY01-02） |
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| 中文摘要: |
| 为探究小麦COL基因在生长发育过程中的潜在功能,本研究采用生物信息学方法在小麦全基因组水平系统分析和鉴定了TaCOL基因,并利用qRT-PCR检测其在不同组织、不同胁迫条件、光照和黑暗处理条件下的表达模式。研究结果显示,在小麦全基因组水平上一共鉴定到48个COL基因,它们在进化关系上被分为I、II、III三组。亚细胞定位预测表明所有TaCOL蛋白均定位在细胞核中。染色体定位分析发现它们不均等地分布在小麦的16条染色体上,其中有4个TaCOL基因组成了4个片段复制基因对。基因结构和保守基序分析发现相同亚组的TaCOL在基因结构和保守基序组成上表现类似,其中Motif 1和Motif 2分别是构成B-box和CCT两个保守结构域的主要基序。此外,在TaCOL基因的启动子区分布着许多光调节、激素响应、生长发育、胁迫响应和其他类型等五种类型顺式作用元件。表达模式分析发现所有的TaCOL基因在小麦的不同组织中均有表达,且大部分基因在叶片中的表达水平较高。多数TaCOL基因响应非生物胁迫和激素的诱导表达,同时不同光周期处理也能显著影响TaCOL基因的表达水平。本研究为进一步研究TaCOL基因在小麦的生长发育和作物遗传改良方面奠定了基础。 |
| 英文摘要: |
| To explore the potential function of wheat COL gene in growth and development, this study used bioinformatics methods to systematically analyze and identify TaCOL genes at the whole-genome level of wheat. The expression patterns of these genes under different tissue types, stress conditions, light and dark treatments were detected by qRT-PCR. The results showed that a total of 48 COL genes were identified in the whole-genome level of wheat, which were divided into three groups of I, II and III in evolutionary relationships. Subcellular localization prediction indicated that all TaCOL proteins were located in the nucleus. Chromosome localization analysis showed that they were unevenly distributed on 16 chromosomes of wheat, and 4 TaCOL genes formed 4 fragment pairs of replication genes. Gene structure and conserved motif analysis found that the TaCOL genes in the same subgroup had similar gene structures and conserved motif compositions, and Motif 1 and Motif 2 were the main motifs that constitute the B-box and CCT conserved domains, respectively. Additionally, many cis-acting elements of five types, including light regulation, hormone response, growth and development, stress response, and other types, were found in the promoter regions of TaCOL genes. Expression pattern analysis showed that all TaCOL genes were expressed in different tissues of wheat, and most genes had higher expression levels in leaves. Most TaCOL genes responded to abiotic stress and hormone induction, and long day or short day treatment could significantly affect the expression levels of TaCOL genes. This study lays a foundation for further research on the function of TaCOL genes in wheat growth and development and crop genetic improvement. |
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