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作者	单位
费新茹，朱娟，郭红，吕超，郭宝健，许如根	（1.扬州大学江苏省作物遗传生理国家重点实验室培育点/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室/扬州大学大麦研究所，江苏扬州 225009； 2.盐都区农业科学研究所，江苏盐城 224011）

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中文摘要:

大麦黄花叶病是依靠土壤中禾谷多黏菌传播的病毒病，严重影响冬大麦的产量和品质，培育和利用抗病品种是最经济有效的防治方法。本研究以抗病品种扬饲麦1号与感病品种Gairdner为亲本，以杂交F₁代经花药离体培养技术构建的DH群体为材料，对其大麦黄花叶病抗性进行两年鉴定与分析，并利用91对在亲本间多态性好的SSR(simple sequence repeat)标记构建了群体的遗传连锁图谱，采用Windows QTL IciMapping 4.0软件中的完备区间-加性模型（ICIM-ADD）对大麦黄花叶病进行QTL定位，共检测到9个与大麦黄花叶病病情指数相关的QTLs，其中，qRYM-1Ha、 qRYM-1Hc、qRYM-2Ha 及qRYM-2Hb在两年间均被检测到，且 qRYM-2Ha在两年6个时期均被检测到，位于EBmag0793至GBM1047标记区间内，可解释的表型变异为5.61%～38.04%； qRYM-2Hb在 2015年第二、三期和2016年第一期被检测到，位于GBM1047至Bmag0749标记区间内，可解释的表型变异为11.40%～18.80%。qRYM-1Ha和 qRYM-4Ha可能是两个新的大麦黄花叶病抗性QTLs，黄花叶病抗性基因均来源于黄花叶病抗性品种扬饲麦1号。本研究为大麦黄花叶病抗性基因的发掘、精细定位、基因克隆及分子标记辅助选择育种提供了有利信息。

英文摘要:

Barley yellow mosaic disease is a kind of virus disease which relies on the transmission of Polymyxa graminis in the soil,seriously decreasing the yield and quality of winter barley. Resistant cultivars are the most cost effective measure for controlling the disease. In this paper,a total of 173 doubled-haploid(DH) lines that generated from a cross between a disease-resistant Chinese landrace barley Yangsimai 1 and a susceptible cultivar barley Gairdner were used. The resistance to barley yellow mosaic disease was identified and analyzed by using DH population and its parents across two years. A genetic linkage map including 91 pairs of SSR markers with obvious polymorphism between the parents was constructed. QTL mapping for barley yellow mosaic disease was carried out by using the complete interval-additive model(Inclusive Composite Interval-Additive Mapping,ICIM-ADD) method in Windows QTL IciMapping 4.0 software. The result showed that nine QTLs for the disease index of barley yellow mosaic disease were identified,of which four QTLs(qRYM-1Ha,qRYM-1Hc,qRYM-2Ha and qRYM-2Hb) were detected across two years. qRYM-2Ha was detected in six stages across two years,and located in the EBmag0793-GBM1047 intervals,which explained 5.61%-38.04% of barley yellow mosaic disease resistant variation; qRYM-2Hb was detected in the second and third stages of 2015 and the first stage of 2016,and located in the GBM1047-Bmag0749 intervals,which explained 11.40%-18.80% of barley yellow mosaic disease resistant variation. Two putative QTLs,qRYM-1Ha and qRYM-4Ha likely represented new loci for barley yellow mosaic disease resistance. Notably,the resistance genes of barley yellow mosaic disease were all derived from the resistant variety Yangsimai 1. The results have supplied valuable information for fine mapping,cloning genes and further molecular-marker-assisted selection breeding of barley yellow mosaic disease.

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