| In order to provide reference for the mutation identification and selection of parents, ISSR markers were used to identify the hybrid F1 generation of new triticale lines P1 and P2.Besides, the genetic diversity of F1 population was studied based on the field investigation results.The results showed that, among 66 F1 plants, 52 were true hybrids, and 46 plants were used as the experimental materials in this study because the rest of six plants did not grow normally.The amplification percentage was 61.19% for 46 F1 hybrid plants while using 14 ISSR primers, among which, amplification percentage was 75% for the primer UBC808 and UBC815, and 100% for the primer UBC822.For 46 F1 plants and their parents, the genetic similarity coefficient ranged from 0.62 to 0.94, which proved that the hybrid offspring produced abundant genetic variation.Cluster analysis showed that the 46 F1 plants and their parents were classified into 3 categories, which were male parent, female parent and their F1 generation, respectively.The F1 generation was firstly clustered with the female and then with the male, which demonstrated that the F1 population had closer relationship with the female parent than that with the male parent.The results of field investigation showed that the F1 population was significantly different from male parent in seven traits, such as the number of tillers and effective tillers, spike length, the number of spikelet and seeds, and grain weight per spike, except plant height, which is consistent with the results of ISSR analysis. |
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