| 莫洪婷，黄新荣.盐胁迫下青稞幼苗生理响应和转录组分析[J].麦类作物学报,2026,(8):1082 |
| 盐胁迫下青稞幼苗生理响应和转录组分析 |
| Physiological Response and Transcriptome Analysis of Highland Barley Seedlings under Salt Stress |
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| DOI： |
| 中文关键词: 青稞幼苗 盐胁迫 生理指标 转录组 加权基因共表达网络分析 |
| 英文关键词:Highland barley seedlings Salt stress Physiologic index Transcriptome Weighted gene co-expression network analysis |
| 基金项目:国家复份库运行服务科技部平台项目（NCGRC-2024-20）；青海大学青年科研基金项目（2023-QNY-8）；青海大学大学生科研训练计划项目（SRT202570） |
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| 中文摘要: |
| 为探究青稞幼苗对盐胁迫的生理与分子响应机制，以高耐盐青稞种质ZYM1795、ZYM3130和不耐盐青稞种质ZYM2239为材料，采用50、150、250 mmol·L-1 NaCl溶液以及蒸馏水（对照）进行处理，测定青稞幼苗叶片中丙二醛（MDA）、可溶性总糖（SS）含量以及过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和过氧化物酶（POD）活性等生理指标，并对对照和250 mmol·L-1 NaCl处理的叶片进行转录组测序。结果表明，随盐浓度的升高，耐盐材料的MDA含量呈先增后减趋势，SOD、POD、CAT活性总体呈先升后降趋势，SS含量显著积累，说明青稞通过激活抗氧化防御系统和渗透调节物质以应对盐胁迫。通过转录组分析，共鉴定出3 343个可能与耐盐性相关的差异表达基因。经加权基因共表达网络分析，筛选出与所有生理指标显著相关的黄色模块，进一步获得1 801个潜在耐盐相关基因。经GO富集分析，这些基因主要富集于抗逆响应、抗氧化防御、激素信号转导，如脱落酸响应、酶活性调控等相关通路。其中，筛选出20个关键基因，包括过氧化物酶基因、海藻糖磷酸化酶基因、丝氨酸/苏氨酸蛋白激酶基因及ABC转运蛋白基因，这些基因在活性氧清除、渗透调节、信号转导中起重要作用，推测青稞幼苗通过协同调控生理响应与关键基因表达以增强耐盐性。 |
| 英文摘要: |
| In order to explore the physiological and molecular response mechanism of highland barley seedlings to salt stress, ZYM1795, ZYM3130 and ZYM2239 highland barley seedlings were used as materials. The seedlings were treated with 50, 150, and 250 mmol·L-1 NaCl solution and distilled water (control). The contents of malondialdehyde(MDA), total soluble sugar(SS) and the activities of catalase(CAT), superoxide dismutase(SOD), and peroxidase(POD) in the leaves of highland barley were determined. Transcriptome sequencing was performed on the leaves of the control and 250 mmol·L-1 NaCl treatment. The results showed that with the increase of salt concentration, the MDA content of salt-tolerant materials increased first and then decreased; the activities of SOD, POD and CAT increased first and then decreased; and the SS content accumulated significantly, indicating that highland barley responded to salt stress by activating antioxidant defense system and osmotic adjustment substances. Through transcriptome analysis, a total of 3 343 differentially expressed genes that may be related to salt tolerance were identified. Through weighted gene co-expression network analysis, yellow modules significantly related to all physiological indicators were screened, and 1 801 potential salt tolerance-related genes were further obtained. GO enrichment analysis showed that these genes were mainly enriched in stress response, antioxidant defense, hormone signal transduction, such as abscisic acid response, enzyme activity regulation and other related pathways. Among them, 20 key genes were screened, including peroxidase gene, trehalose phosphorylase gene, serine/ threonine protein kinase gene, and ABC transporter gene. These genes play an important role in active oxygen scavenging, osmotic regulation, and signal transduction. It is speculated that highland barley seedlings enhance salt tolerance by synergistically regulating physiological response and key gene expression. |
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