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作者	单位
崔东凯，刘可可，程文聪，韩思蕊，邓璐，刘夏燕，刘振山，秦锦霞	（1.西北农林科技大学未来农业研究院,陕西杨凌 712100；2.西北农林科技大学生命科学学院,陕西杨凌 712100；3.西北农林科技大学农学院,陕西杨凌 712100）

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中文摘要:

为探究高温下小麦全基因组水平mRNA二级结构重编程及其与翻译效率的关联，以小麦品种中国春为材料，利用SHAPE MaP及多聚核糖体图谱技术，在不同条件下对苗期小麦进行了多组学联合分析。结果表明，小麦转录本起始与终止密码子位置的SHAPE反应活性显著高于两侧，呈现保守结构特征。与常温相比，高温胁迫导致全基因组转录本平均SHAPE反应活性降低，mRNA倾向于形成双链结构，且该变化在恢复期表现出可逆性。高温胁迫初期，起始密码子区域的结构变化最为显著。多聚核糖体图谱显示高温导致多聚核糖体峰值下降，全基因组水平的翻译受到抑制。经关联分析，全基因组水平上转录本各区域的SHAPE反应活性变化与翻译效率变化之间无显著相关性。综上所述，高温诱导小麦mRNA折叠程度增加可能是一种主动防御策略，其与翻译调控呈现非线性复杂关系。

英文摘要:

To investigate genome-wide mRNA secondary structure reprogramming and its correlation with translation efficiency under heat stress in wheat, this study performed a multi-omics analysis on wheat seedlings(cv. Chinese Spring) under different conditions, utilizing SHAPE-MaP and polysome profiling. The results showed that the SHAPE reactivity of start and stop codon positions in wheat transcripts was significantly higher than that of flanking regions, exhibiting conserved structural features. Compared with the control, heat stress resulted in a decrease in the average SHAPE reactivity across transcripts, indicating that mRNA tends to form double-stranded structures. This change exhibited reversibility during recovery. At the early stage of heat stress, structural changes in the start codon region were the most significant. Polysome profiling showed that heat stress caused a decrease in polysome peaks, and translation was inhibited at the genome-wide level. Correlation analysis revealed that there was no significant correlation between SHAPE reactivity changes and translation efficiency changes of various functional regions at the genome-wide level. In summary, the heat-induced increase in mRNA folding may be a proactive defense strategy, which presents a non-linear complex relationship with translational regulation.

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