| 王长杰，汪欣瑶，陈佳佳，赵 娜，王勇胜，牛 丽，张 宏，姚立蓉，司二静，杨 轲，孟亚雄，马小乐，王化俊，汪军成.大麦R2R3-MYB转录因子家族鉴定及低磷胁迫下Hv2RMYB-48的功能验证[J].麦类作物学报,2026,(8):1000 |
| 大麦R2R3-MYB转录因子家族鉴定及低磷胁迫下Hv2RMYB-48的功能验证 |
| Identification of the R2R3-MYB Transcription Factor Family in Barley and Functional Verification of Hv2RMYB-48 under Low-Phosphorus Stress |
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| DOI： |
| 中文关键词: 大麦 R2R3-MYB转录因子 低磷胁迫 生物信息学 酵母单杂交 |
| 英文关键词:Barley R2R3-MYB transcription factor Low phosphorus stress Bioinformatics Yeast one-hybrid analysis |
| 基金项目:财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05-02A-02);甘肃省现代农业产业技术体系建设项目（GSARS07）;甘肃农业大学创新训练项目（202501076） |
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| 中文摘要: |
| R2R3-MYB转录因子家族是MYB转录因子中最大亚家族，在植物多种胁迫响应中扮演重要角色。为了筛选出与磷利用效率相关的R2R3-MYB成员，以最新组装的大麦MorexV3基因组对大麦中的R2R3-MYB转录因子家族进行了鉴定与生物信息学分析。结果表明，在大麦MorexV3基因组中鉴定出127个R2R3-MYB基因，并命名为Hv2RMYB-1~Hv2RMYB-127。这些基因分布于大麦7条染色体上，且启动子区富含激素响应顺式作用元件。经理化性质分析，大多数成员编码酸性且不稳定的蛋白质。通过系统发育及共线性分析将大麦R2R3-MYB家族划分为5个不同的分支及16对串联复制基因。 结合转录组数据（RNA-Seq）和实时荧光定量PCR技术（qRT-PCR），发现10个大麦R2R3-MYB基因在低磷胁迫下均有不同程度的差异性表达，其中Hv2RMYB-48显著上调表达。Hv2RMYB-48定位于细胞核，可以与MYB-ABRE-MYB顺式作用元件进行互作。 |
| 英文摘要: |
| The R2R3-MYB transcription factor family, the largest subfamily of MYB transcription factors, plays critical roles in various plant stress responses. However, its function under low-phosphate(P) stress remains poorly characterized. In this study, a total of 127 R2R3-MYB genes were identified in the barley MorexV3 genome using bioinformatic approaches and designated as Hv2RMYB-1 to Hv2RMYB-127. These genes were predominantly distributed on the 7 barley chromosomes and were found to harbor hormone-responsive cis-acting elements in their promoter regions. Physicochemical analysis revealed that most members encode acidic and unstable proteins. Phylogenetic and collinearity analyses classified the barley R2R3-MYB family into five distinct clades, including 16 pairs of tandemly duplicated genes. Based on transcriptomic data(RNA-Seq) and quantitative real-time PCR(qRT-PCR), 10 barley R2R3-MYB genes showed differential expression under low-Phosphorus stress, among which Hv2RMYB-48 was notably upregulated. Further investigation confirmed that Hv2RMYB-48 localizes to the nucleus and can bind to the MYB-ABRE-MYB cis-element. |
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