| 杜迪迪，吴保为，龚 悦，马 猛，刘香利，赵惠贤.敲除TaMAPK1部分同源基因对小麦苗期耐旱性的调控机制[J].麦类作物学报,2026,(8):991 |
| 敲除TaMAPK1部分同源基因对小麦苗期耐旱性的调控机制 |
| Regulatory Mechanism Underlying Drought Tolerance in Wheat Seedlings via TaMAPK1 Partial Homeologous Genes Knockout |
| |
| DOI： |
| 中文关键词: 小麦 TaMAPK1 耐旱性 基因编辑 同源基因 叠加效应 |
| 英文关键词:Wheat TaMAPK1 Drought tolerance Gene editing Homoeologs Additive effect |
| 基金项目:陕西省重点研发项目（2025NC-YBXM-002）；国家自然科学基金项目（32372103和32072003）;陕西省自然科学基础研究计划项目（2024JC-YBMS-171） |
|
| 摘要点击次数: 178 |
| 全文下载次数: 47 |
| 中文摘要: |
| 为解析小麦丝裂原活化蛋白激酶基因TaMAPK1-6A、TaMAPK1-6B和TaMAPK1-6D在苗期耐旱性中的功能及其同源基因之间的叠加效应，以春小麦品种Fielder及以其为遗传背景通过CRISPR/Cas9介导的小麦基因组编辑技术创制的TaMAPK1单基因突变体mapk1-aa、mapk1-bb和mapk1-dd（简称单突变体）为供试材料，通过杂交、自交及其后代分离群体的基因型检测，创制了双基因敲除突变体和三基因敲除纯合突变体（分别简称双突变体和三突变体），并对这些单突变体、双突变体和三突变体以及对照（野生型Fielder）苗期耐旱性进行了系统评价。结果表明，TaMAPK1单基因功能缺失突变导致小麦幼苗耐旱性显著降低，表现为叶片保水能力下降、渗透调节物质积累减少和膜脂过氧化加剧。双突变体和三突变体的耐旱性较单突变体进一步降低，生理生化指标恶化程度随突变基因拷贝数增加而加剧。综上，TaMAPK1基因正调控小麦苗期耐旱性，其同源基因具有累加调控作用。 |
| 英文摘要: |
| To elucidate the functions of the wheat mitogen-activated protein kinase genes TaMAPK1-6A, TaMAPK1-6B, and TaMAPK1-6D in seedling drought tolerance and the additive effects among these homeologous genes, this study utilized the spring wheat cultivar Fielder and its CRISPR/Cas9 mediated single-gene mutants-mapk1-aa, mapk1-bb, and mapk1-dd-generated in the Fielder genetic background. Through hybridization, self-pollination, and genotyping of segregating progeny, double-gene knockout mutants and triple-gene knockout homozygous mutants(hereafter referred to as double and triple mutants) were successfully developed. The drought tolerance at the seedling stage was systematically evaluated for these single, double, and triple mutants, along with the wild-type Fielder control. The results demonstrated that loss-of-function mutations in single TaMAPK1 genes significantly reduced drought tolerance in wheat seedlings, manifested as decreased leaf water retention, reduced accumulation of osmotic adjustment substances, and enhanced membrane lipid peroxidation. Further investigation revealed that drought tolerance was further impaired in double and triple mutants, with the deterioration of physiological and biochemical indicators intensifying as the number of mutated gene copies increased. In summary, the TaMAPK1 gene positively regulates drought tolerance at the wheat seedling stage, and its homeologous genes exhibit cumulative regulatory effects. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
基本信息
