| 姜 雪，张裕梁，倪春阳，董永文，颜 磊，赵 灿，许 轲，王维领，霍中洋.5 氨基乙酰丙酸对盐胁迫下小麦种子萌发生长的影响及其生理机制分析[J].麦类作物学报,2026,(1):130 |
| 5 氨基乙酰丙酸对盐胁迫下小麦种子萌发生长的影响及其生理机制分析 |
| Effect of 5-Aminolevulinic Acid on Seed Germination and Growth of Wheat under Salt Stress and Analysis of Its Physiological Mechanism |
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| DOI： |
| 中文关键词: 盐胁迫 5-氨基乙酰丙酸 小麦 种子萌发 生理机制 |
| 英文关键词:Salt stress 5-aminolevulinic acid Wheat Seed germination Physiological mechanism |
| 基金项目:国家自然科学基金（32301937）；扬州市绿扬金凤人才计划资助项目（YZLYJFJH2021YXBS149）；江苏高校优势学科建设工程资助项目（PAPD） |
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| 中文摘要: |
| 5-氨基乙酰丙酸（5-ALA）作为一种新型的植物生长调节剂，在缓解植物非生物胁迫方面广泛应用。为了明确5-ALA浸种处理改善小麦种子耐盐性的适宜浓度及其生理机制，以小麦品种扬麦25为材料，比较分析不同浓度5-ALA（0、5、10、25、50、100 mg·L-1）浸种处理对盐胁迫（100 mmol·L-1氯化钠溶液）下小麦种子萌发生长、物质活化、能量供应、离子与氧化还原稳态等方面的影响。结果表明，5~100 mg·L-1的5-ALA浸种处理显著缩短盐胁迫下小麦种子的平均发芽时间（0.23~0.42 d），明显促进了胚根和胚芽的生长；25 mg·L-1的5-ALA浸种处理提高了小麦种子的吸胀速率及盐胁迫下小麦淀粉酶、6-磷酸果糖激酶、己糖激酶活性，呼吸速率和三磷酸腺苷含量，同时显著降低了小麦种子总淀粉和可溶性总糖含量；5-ALA浸种处理未能缓解盐胁迫下小麦种子Na+的过量积累，但有效抑制了盐胁迫引起的K+外流;5-ALA浸种处理显著降低了盐胁迫下小麦种子丙二醛的含量。由此可见，5-ALA浸种处理主要通过加速贮藏淀粉降解，维持能量供应水平以及离子和氧化还原稳态，以促进盐胁迫下小麦种子的萌发与生长。 |
| 英文摘要: |
| 5-aminolevulinic acid(5-ALA), as a novel plant growth regulator, has been widely applied in alleviating abiotic stress in plants. In this study, a wheat cultivar Yangmai 25 was used as the experimental material to investigate the effects of seed soaking with different concentrations of 5-ALA(0, 5, 10, 25, 50, and 100 mg·L-1) on seed germination, material activation, energy supply, as well as ionic and redox homeostasis under salt stress simulated by 100 mmol·L-1 NaCl solution. The objectives were to identify the optimal concentration of 5-ALA for enhancing salt tolerance in wheat seeds and to elucidate its physiological mechanisms. The results demonstrated that seed soaking with 5-ALA(5-100 mg·L-1) markedly shortened the mean germination time of wheat seeds under salt stress by 0.23-0.42 days and significantly promoted the growth of both radicle and coleoptile. Further analysis revealed that 25 mg·L-1 5-ALA priming increased wheat swelling rates, as well as the activities of amylase, 6-phosphofructokinase, and hexokinase under salt stress, along with enhanced respiration rates and adenosine triphosphate(ATP) content, while significantly reducing starch and soluble sugar contents in wheat. Although 5-ALA priming did not mitigate excessive Na+ accumulation in wheat under salt stress, it effectively inhibited K+ loss induced by salt stress. Moreover, 5-ALA priming significantly reduced malondialdehyde(MDA) accumulation in wheat under salt stress. These findings suggest that 5-ALA seed priming promotes wheat seed germination and growth under salt stress by accelerating starch degradation, maintaining energy supply levels, and preserving ionic and redox homeostasis. |
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