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作者	单位
王孜逸，李嘉伟，耿梓瀚，倪守飞，丛钰莹，刘浩，赵彦宏，胡德昌，王艳芳	(1.鲁东大学生命科学学院，山东烟台 264025 2. 鲁东大学园艺学院，山东烟台 264025)

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中文摘要:

GATA基因家族成员是植物重要的转录因子，广泛参与植物生长发育及多种生理生化过程。为了解GATA家族成员在大麦中的分布及其功能，基于生物信息学方法系统开展了大麦GATA基因家族成员的全基因组鉴定及其结构、共线性、启动子、表达模式等分析。结果表明，在大麦基因组中共鉴定出27个GATA基因，依次命名为HvGATA01~HvGATA27，其不均匀分布于7 条染色体上。27个HvGATA基因的内含子数在0～7之间，所有成员均包含典型的GATA保守结构域。通过系统进化分析将27个成员划分为5个亚组（S1~S5），其中S5亚组包含9个成员，S3亚组仅1个成员；不同亚族成员间的内含子和外显子分布存在明显差异，且除S3亚组外，其余亚组成员均在单、双子叶植物分化前已存在。经共线性分析，在大麦与水稻、拟南芥、二穗短柄草、大豆、番茄基因组间均存在共线性GATA基因对，但大麦与水稻、二穗短柄草中的共线性基因对明显多于其余三个物种。通过顺式作用元件分析，大麦GATA启动子区富集有光响应元件及激素信号响应元件。HvGATA基因在大麦16 个不同组织中的表达存在明显差异，多数HvGATA基因在花序中呈现高表达特征，而在去苞片籽粒中表达量较低，暗示其可能参与大麦开花发育调控。

英文摘要:

The GATA gene family is a crucial class of transcriptional regulators in plants, widely involved in plant growth, development, and various physiological and biochemical processes. In this study, a comprehensive genome-wide analysis of the barley GATA transcription factor family was performed using bioinformatics approaches, including gene identification, structural characterization, collinearity analysis, promoter element prediction, and expression pattern profiling. The results showed that the barley genome contains a total of 27 GATA genes(designated as HvGATA01-HvGATA27), which were unevenly distributed across the 7 chromosomes. Gene structure analysis revealed that the number of introns in HvGATA genes ranged from 0 to 7, with distinct intron-exon arrangements observed among different subgroups. All members contain the typical GATA conserved domain. Phylogenetic analysis divided them into five subgroups(S1-S5), of which subgroup S5 contains 9 members, while subgroup S3 has only one member. Except for S3, the remaining subgroups existed before the divergence of monocots and dicots. Collinearity analysis revealed that collinear GATA gene pairs exist between the barley genome and those of rice, Arabidopsis thaliana, Brachypodium distachyon, soybean, and tomato. Notably, the number of collinear gene pairs in barley-rice and barley-Brachypodium distachyon comparisons was significantly higher than that in the other three species. Cis-acting element analysis indicated that the promoter regions of barley GATA genes are enriched with light-responsive elements and hormone signaling-related elements. Expression profiling showed significant differential expression patterns across 16 barley tissues, with most HvGATA genes exhibiting high expression in inflorescence tissues, while low expression was observed in deglumed grains, suggesting their potential involvement in barley flowering development regulation.

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