| 郭小玲,俞天胜，汪永法，程宇坤，任 毅，张晓玉，耿洪伟.冬小麦RIL群体分蘖相关性状QTL定位[J].麦类作物学报,2026,(1):58 |
| 冬小麦RIL群体分蘖相关性状QTL定位 |
| QTL Mapping and Candidate Gene Screening of Tillering-Related Traits in RIL Populations of Winter Wheat |
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| DOI： |
| 中文关键词: 小麦 分蘖性状 超低深度测序 QTL分析 |
| 英文关键词:Wheat Tillering traits Ultra-low depth sequencing QTL analysis |
| 基金项目:新疆小麦产业技术体系项目（XJARS-01-02）; 庭州科技创新团队（引领型）项目（2023CT01） |
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| 中文摘要: |
| 为进一步挖掘小麦分蘖相关性状的数量性状位点（QTL），探索分蘖性状之间的遗传关系，以分蘖性状差异较大的小麦品种Apache和新冬36号构建的 255 份重组自交系群体（RIL）为材料，分别测定其在 4个环境下的冬前分蘖数、单株分蘖数、单株有效分蘖数、单位面积有效分蘖数、分蘖角度、成穗率表型值，并基于 RIL 群体的超低深度测序数据进行分蘖相关性状 QTL 定位。结果表明，除 1D、2D、3B、4D、7A和7B 染色体外，在其他 15 条染色体上共检测到 53个分蘖性状相关的 QTL，包括 13个冬前分蘖数 QTL、10个单株分蘖数 QTL、8个单株有效分蘖数 QTL、11个单位面积有效分蘖数 QTL、4个分蘖角度 QTL、2个成穗率 QTL和5个一因多效的QTL，单个QTL 的表型解释率为 2.66％～28.76％。其中，QTn.xjau-4B在3个环境下均被检测到， 对单株有效分蘖数表型贡献率为28.76%，为稳定主效 QTL,同时也是R-E-Z位点，后续可作为研究分蘖及株型的重要位点。 |
| 英文摘要: |
| In order to further explore the quantitative trait locus(QTL) for tillering-related traits in wheat, and to explore the genetic relationship between tillering traits, we used 255 recombinant inbred lines(RIL) populations, constructed from Apache and Xindong 36, as research materials to determine several key metrics. These included the number of pre-winter tillers, the number of single tillers, the number of effective tillers, the effective tillers per unit area, and the tiller angle. The phenotypic values of spike rate were determined in four environments. The QTL controlling tillering-related traits was carried out based on the ultra-low depth sequencing data of the RIL populations. The results showed that a total of 53 QTLs for tillering traits were detected on 15 chromosomes, including 13 QTLs for pre-winter tiller number, 10 QTLs for single-plant tiller number, 8 QTLs for effective tiller number per plant, 11 QTLs for effective tiller number per unit area, 4 QTLs for tiller angle, 2 QTLs for spike-forming rate, and 5 pleiotropism QTLs. The phenotypic interpretation rates of individual QTLs ranged from 2.66% to 28.76%. Among them, QTn.xjau-4B was detected in all three environments, with a phenotypic contribution rate of 28.76% to the effective number of tillers of a single plant. This stable QTL is a major effective and R-E-Z locus, which can be used as an important locus for the study of tillering and plant type. |
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