| 张娇梦，刘 源，高 璞，王新海，张培培，李在峰.Kenya Kudu/郑州5389 F2∶3群体成株期抗叶锈基因QTL定位[J].麦类作物学报,2026,(1):51 |
| Kenya Kudu/郑州5389 F2∶3群体成株期抗叶锈基因QTL定位 |
| QTL Mapping of Adult-Plant Resistance to Leaf Rust Genes in the Kenya Kudu/Zhengzhou 5389 F2∶3 Population |
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| DOI： |
| 中文关键词: 小麦 叶锈病 成株期抗性 QTL定位 |
| 英文关键词:Wheat Leaf rust Adult plant resistance QTL mapping |
| 基金项目:国家重点研发计划项目（2023YFD1201002-02）;国家自然科学基金项目（32161143007）；河北省高等学校科学研究项目（BJ2025101） |
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| 中文摘要: |
| 肯尼亚小麦品种Kenya Kudu在田间表现良好的成株期抗叶锈性。为解析Kenya Kudu的叶锈病抗性遗传基础，以Kenya Kudu与感病品种郑州5389杂交、自交获得的195个F2∶3家系为材料，于2017—2018和2018—2019年度分别在河北保定和河南周口试验田种植，并接种强毒力叶锈菌混合小种进行成株期抗叶锈病鉴定；利用660K SNP芯片数据结合SSR标记和田间表型数据进行QTL分析及定位。结果表明，在小麦2B和5B染色体上共鉴定到3个成株期抗叶锈QTL；其中2个QTL位于5B染色体，暂命名为QLr.hbau-5B.1和QLr.hbau-5B.2，分别解释了8.3%~10.5%和6.9%~8.6%的表型变异；1个QTL位于2B染色体，暂命名为QLr.hbau-2B，解释了7.2%~10.6%的表型变异。3个位点均为微效成株期抗叶锈QTL，说明Kenya Kudu的叶锈病抗性是多个QTLs的累加效应。所鉴定到的QTL可为小麦抗叶锈病育种提供基因资源，与抗病基因紧密连锁的分子标记可辅助选择抗病品种。 |
| 英文摘要: |
| Kenya Kudu, a Kenyan wheat variety, exhibits robust adult plant resistance(APR) to leaf rust. To dissect the genetic architecture underlying this resistance, we conducted quantitative trait locus(QTL) mapping using 195 F2∶3 lines derived from a cross between Kenya Kudu and the susceptible cultivar Zhengzhou 5389. Field evaluations were carried out in Baoding, Hebei Province and Zhoukou, Henan Province during the 2017-2018 and 2018-2019 growing seasons. Plants were inoculated with a mixture of virulent Puccinia triticina(Pt) races to assess APR. Phenotypic data on disease severity were integrated with genotypic data from 660K SNP array and SSR markers for QTL analysis. The results demonstrated that three novel adult plant resistance QTLs to leaf rust were identified on wheat chromosomes 2B and 5B. Two QTLs were located on chromosome 5B, provisionally designated as QLr.hbau-5B.1 and QLr.hbau-5B.2, accounting for 8.3~10.5% and 6.9~8.6% of the phenotypic variation, respectively. One QTL located on chromosome 2B, provisionally designated QLr.hbau-2B, accounted for 7.2~10.6% of the phenotypic variation and may correspond to a known leaf rust resistance locus. These results demonstrate that Kenya Kudus resistance is attributed to the additive effects of multiple minor-effect QTLs. The identified QTLs and their closely linked molecular markers offer critical genetic tools for marker-assisted selection(MAS) in breeding wheat cultivars with durable resistance to leaf rust. |
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