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作者	单位
李丽敏，刘雯，朱娜，郑佳乐，母学荣，刘洋，田业松，张小红，闵东红	（1.西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室，陕西杨凌 712100； 2.西北农林科技大学生命科学学院，陕西杨凌 712100）

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中文摘要:

株高对小麦产量有重要影响。为进一步挖掘小麦株高的数量性状位点（QTL），以矮秆突变体20DH2-2和高秆材料21Hs-7构建的F₂群体为材料，用主基因+多基因混合遗传模型对株高进行遗传分析；利用620对简单重复序列（SSR）分子标记筛选结果构建遗传连锁图谱，结合F₂群体株高表型数据进行QTL定位分析。结果表明，株高的最适遗传模型为2MG-EA（两对等加性主基因）。通过分离群体分组分析法（BSA法）筛选到26对具有多态性的SSR分子标记，构建了全长为531.62 cM遗传连锁图谱。共检测到5个株高相关QTL，分布在2A（2个）、4A、5A及6A染色体上，对数优势比（LOD值）为2.60~8.19，可解释7.97%~22.88%的表型变异。QPH-nwafu-2A.2、QPH-nwafu-4A、QPH-nwafu-5A及QPH-nwafu-6A分别与分子标记BQ838266（距离为4.01 cM）、Xwmc262（距离为3.00 cM）、Xwmc524（距离为3.94 cM）及Xbarc107（距离为0.69 cM）紧密连锁。

英文摘要:

Plant height is a key trait in the growth and development of wheat and has an important impact on wheat yield. To further identify quantitative trait loci(QTL) associated with plant height in wheat, this study utilized an F₂ population derived from a cross between a dwarf mutant 20DH2-2 and a tall parent 21Hs-7. Genetic analysis of plant height was conducted using a mixed dominant gene plus multi-gene inheritance model. A genetic linkage map was constructed using the screening results of 620 simple sequence repeat(SSR) molecular markers, and QTL mapping analysis was performed in combination with the plant height phenotypic data of the F₂ individuals. Results showed that the optimal genetic model for plant height was 2MG-EA(two equal additive major genes). Through bulked segregant analysis(BSA), 26 polymorphic SSR markers were identified, constructing a genetic linkage map spanning 531.62 cM. Five plant height-related QTLs were detected on chromosomes 2A(two QTLs), 4A, 5A, and 6A, with logarithm of odds(LOD scores) ranging from 2.60 to 8.19 and explaining 7.97%~22.88% of phenotypic variation. Specifically, QPH-nwafu-2A.2, QPH-nwafu-4A, QPH-nwafu-5A, and QPH-nwafu-6A tightly linked to molecular markers BQ838266(4.01 cM), Xwmc262(3.00 cM), Xwmc524(3.94 cM), and Xbarc107(0.69 cM), respectively.

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