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作者	单位
孙皓，赵斌，彭严春，王瑞，季昌好，朱斌，陈晓东	（1.安徽省农业科学院作物研究所，安徽合肥 230031； 2.粮食作物种质创新与遗传改良湖北省重点实验室，湖北武汉 430064；3.农作物品质改良安徽省重点实验室，安徽合肥 230031； 4.湖北省农业科学院粮食作物研究所，湖北武汉 430064）

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中文摘要:

多个小麦矮秆(reduced height, Rht)基因已被鉴定，但仅有少数应用于育种，挖掘可利用的新矮源基因仍是培育高产、稳产小麦品种的前提和基础。以优异矮秆小麦新种质11003为研究对象，与长江中下游主推品种扬麦20组配构建F₂分离群体，对株高性状进行遗传分析。结果表明，11003的矮秆表型由1对隐性主效基因控制，混池分离测序分析(BSA-seq)将其矮秆基因定位于4D染色体16.64~25.97 Mb的物理区间内，命名为QPh.aaas-4D。通过KASP标记不断加密，将QPh.aaas-4D精细定位至18.95~21.62 Mb的2.67 Mb候选区间内。经矮秆基因Rht2功能标记KP-Rht2检验，推测QPh.aaas-4D目的基因可能为该经典矮秆基因。基于上述结果，小麦新种质11003株高下降可能与4D染色体上携带Rht2基因有关，以其作亲本组配，可改良小麦株高性状。

英文摘要:

Multiple reduced height(Rht)genes in wheat had been identified but only a few were applied in breeding programs. Therefore, identifying and utilizing new Rht genes remains a prerequisite for breeding high-yield and stable wheat varieties. In this study, we constructed an F₂ mapping population by crossing an elite dwarf wheat germplasm 11003 with Yangmai 20, a leading variety in the middle and lower reaches of the Yangtze River. Genetic analysis indicated that the lower plant height of 11003 was controlled by a pair of recessive major alleles. The Rht gene, designated as QPh.aaas-4D, was mapped to a physical interval of 16.64-25.97 Mb on chromosome 4D by using bulked-segregant analysis sequencing(BSA-Seq). Subsequently, high-density KASP makers were developed and mapped the QPh.aaas-4D to a 2.67 Mb candidate interval(18.95-21.62 Mb). Then we used the functional marker KP-Rht2 to test individuals of the F₂ population and assumed the candidate gene underlying QPh.aaas-4D was the classical Rht gene, Rht2. Based on this study, reduced height of the novel wheat germplasm 11003 is likely related to the Rht2 gene located on chromosome 4D, which can be used in wheat breeding.

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