| 李春春，曾孟柯，刘佳乐，郭 靖，朱志伟，尹一凡，曹琴琴，吴建辉，郑炜君，康振生，韩德俊，曾庆东，李春莲.小麦抗感假禾谷镰孢菌侵染的差异表达基因分析[J].麦类作物学报,2025,(12):1589 |
| 小麦抗感假禾谷镰孢菌侵染的差异表达基因分析 |
| Analysis of Differential Expression Genes in Wheat Varieties with Resistance and Susceptibility to Fusarium pseudograminearum |
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| DOI： |
| 中文关键词: 小麦 小麦茎基腐病 假禾谷镰孢菌 转录组 差异表达基因 |
| 英文关键词:Wheat Fusarium crown rot Fusarium pseudograminearum Transcriptome Differentially expressed genes |
| 基金项目:国家重点研发计划项目（2023YFD1200400） |
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| 摘要点击次数: 127 |
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| 中文摘要: |
| 小麦茎基腐病严重威胁着小麦产量和粮食安全，挖掘抗茎基腐病基因、解析抗病机制对小麦抗病育种具有重要意义。为探究假禾谷镰孢菌（Fusarium pseudograminearum）侵染后小麦防御反应机制，利用转录组测序技术，对前期鉴定筛选出病情指数抗感差异显著的小麦品种瑞华麦520（DI=37.67）与和尚头（DI=93.35）响应假禾谷镰孢菌侵染及差异表达基因进行了分析。结果表明，随着病原菌侵染时间延长，两个品种的差异表达基因数量均持续增加，并且瑞华麦520的差异基因数量更多。通过GO富集分析，接种后两个品种在几丁质酶、谷胱甘肽代谢过程和O-甲基转移酶活性等通路上均显著富集，说明细胞壁成分、植物激素以及氧化还原过程等相关基因在抗茎基腐病中发挥着关键作用，这些通路在抗感品种间富集的基因数量存在差异；经KEGG分析，瑞华麦520中富集的前20条通路中有13条与和尚头相同，且绝大多数通路中的基因数量更多，推测瑞华麦520可能通过调控防御物质合成、氧化还原状态和免疫反应等通路，从而提高了对茎基腐病的抗性。 |
| 英文摘要: |
| Fusarium crown rot, caused by Fusarium pseudograminearum(Fp), frequently occurs and severely threatens wheat yield and food security. Identifying resistance genes and elucidating the resistance mechanisms are crucial for breeding resistant varieties. To investigate the defense response mechanisms of wheat to Fp infection, this study employed RNA sequencing to analyze the differentially expressed genes(DEGs) in varieties Ruihuamai 520(DI=37.67) and Heshangtou(DI=93.35). The results showed that the number of DEGs in both varieties increased continuously with the duration of fungal infection, with a significantly higher number observed in the resistant variety Ruihuamai 520. Gene Ontology(GO) enrichment analysis revealed that chitinase activity, glutathione metabolism, and O-methyl transferase activity were significantly enriched in both varieties after inoculation. These findings indicate that genes related to cell wall composition, phytohormones, and redox processes play key roles in the defense response against Fp. Notably, the number of enriched genes in these pathways differed between resistant and susceptible varieties. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathyway analysis showed that 13 of the top 20 enriched pathways in Ruihuamai 520 were also enriched in Heshangtou. However, the number of genes involved in most of these pathways was greater in Ruihuamai 520. These findings suggest that Ruihuamai 520 may enhance resistance to Fp by regulating of pathways involved in the synthesis of defense compounds, maintenance of redox homeostasis, and activation of immune responses. |
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