| 吴 迪，胡文静，吴旭江，张晓祥，江 伟，高致富，高德荣.HGGT基因对减少小麦籽粒DON毒素积累的效应解析及低毒素种质筛选[J].麦类作物学报,2025,(11):1503 |
| HGGT基因对减少小麦籽粒DON毒素积累的效应解析及低毒素种质筛选 |
| Analysis of the Effect of HGGT Gene on Reducing the Accumulation of DON Toxin in Wheat Grains and Screening of Low Toxin Germplasm |
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| DOI： |
| 中文关键词: 关键词：小麦 赤霉病 HGGT基因 标记开发 DON毒素 育种利用 |
| 英文关键词:Wheat Fusarium head blight HGGT gene Marker development DON toxin Breeding utilization |
| 基金项目:江苏省农业科技自主创新资金项目（CX(23)3089）；国家自然科学基金项目（32472100）；江苏里下河地区农业科学研究所青年基金专项（SJ(24)04） |
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| 中文摘要: |
| 小麦感染赤霉病后籽粒中会有脱氧雪腐镰孢菌醇（DON）毒素产生，影响小麦品质和人畜健康。在小麦中，DON毒素的毒性主要通过氧化应激引起，植物中的维生素E主要作用是抗氧化，而尿黑酸牻牛儿基牻牛儿基转移酶基因HGGT是维生素E合成的关键限速基因。为了验证HGGT基因是否存在降低小麦籽粒毒素积累的效应，利用自开发的HGGT基因特异性标记对133份中国主推品种及部分地方品种组成的自然群体进行检测，筛选出携带HGGT基因的品种23份，并对这些品种两年平均病小穗率及籽粒DON毒素含量数据进行关联分析。结果表明，携带HGGT基因的品种平均病小穗率为20.47%，比不携带该基因的品种平均病小穗降低22.63个百分点（P<0.01），说明HGGT基因在小麦赤霉病的扩展抗性方面也存在一定的效应。在未接种条件下，携带HGGT基因的品种平均籽粒DON毒素含量为0.12 mg·kg-1，比不携带该基因的品种平均籽粒DON毒素含量降低75.70%（P<0.05）；在接种条件下，携带HGGT基因的品种平均籽粒DON毒素含量为5.51 mg·kg-1, 比不携带该基因的品种平均籽粒DON毒素含量降低52.97%（P<0.01）。随后将自然群体接种后与接种前籽粒DON毒素含量差值与基因型数据关联分析，发现携带HGGT基因与不携带HGGT基因的品种之间籽粒DON毒素含量存在极显著差异（P<0.01），携带该基因的毒素积累降低51.93%，验证了HGGT基因对小麦籽粒DON毒素积累存在显著的负调控效应。利用特异性标记在自然群体中筛选出了携带HGGT基因的扬麦18、扬麦33、苏麦3号等小麦品种，这些品种接种后两年平均籽粒DON毒素含量均低于国标GB2761-2011限量标准（1 000 μg·kg-1），可作为亲本用于小麦低DON毒素新种质创制。 |
| 英文摘要: |
| Fusarium head blight is a worldwide disease caused by Fusarium graminea, which seriously affects the yield and quality of wheat. The toxin of deoxynivetol(DON) produced by the grain infected by the pathogen poses a serious threat to human and animal health. In wheat, the toxicity of DON toxin is mainly caused by oxidative stress, and the main function of vitamin E in plants is antioxidant. In order to verify the effect of HGGT gene on reducing toxin accumulation in wheat grains, we developed the specific markers for HGGT gene and screened 23 varieties carrying HGGT gene from 133 national dominant varieties and some local varieties and lines. Combined with the data of two-year average diseased spikelet rate and grain DON toxin content, the correlation analysis showed that: the average diseased spikelet rate with HGGT gene was 20.47%, which was 22.67 percentage points lower than that without HGGT gene(P<0.01), indicating that HGGT gene also had a certain effect on the spread resistance of wheat scab. Under the conditions of no inoculation, the average grain DON toxin content of cultivars carrying HGGT gene was 0.12 mg·kg-1, which was 75.70% lower than that of cultivars without HGGT gene(P<0.05). Under inoculation conditions, the average grain DON toxin content of cultivars carrying HGGT gene was 5.51 mg·kg-1, which was 52.97% lower than that of cultivars without HGGT gene(P<0.01). The difference of DON toxin content in grains after inoculation and before inoculation was correlated with genotype data, and it was found that there was a significant difference in the changes of toxin content in grains between varieties carrying HGGT gene and those without HGGT gene(P<0.01), and the DON toxin accumulation with this gene was reduced by 51.93%. It was verified that HGGT gene had a significantly negative regulatory effect on the accumulation of DON toxin in wheat grains. Yangmai 18, Yangmai 33, Sumai 3, harboring HGGT, had the lower grain DON toxin content than the national standard GB2761-2011(1 000 μg·kg-1) based on the phenotype of two years. This study could promote development of new wheat germplasm with low DON toxin. |
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