| 马 新，王 娟，姚丹妤，万小能，吕凯迪，杨柏松，王 含，杨淑颖，郝淑女，张慧慧，陈彦竹，王 彩，马守才，孙果忠.小麦幼穗在减数分裂期响应低温胁迫的转录组分析[J].麦类作物学报,2025,(11):1451 |
| 小麦幼穗在减数分裂期响应低温胁迫的转录组分析 |
| Transcriptome Analysis of Wheat Young Spikes in Response to Low Temperature Stress at Meiosis Stage |
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| DOI： |
| 中文关键词: 小麦 减数分裂期 低温 转录组 |
| 英文关键词:Triticum aestivum L. Meiosis stage Low temperature Transcriptome |
| 基金项目:国家自然科学基金项目(32201806)；中国农业科学院创新工程项目(CAAS-ZDRW202002)；国家重点研发计划项目(2021YFD12006)；财政部和农业农村部国家现代农业产业技术体系项目（CARS-03）；科技创新2030-重大项目（2023ZD0402402） |
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| 中文摘要: |
| 由春季低温引发的倒春寒灾害在中国频繁发生，解析小麦幼穗应答低温胁迫的基因调控机制对抗倒春寒遗传改良具有重要意义。在人工控制环境条件下，通过转录组测序技术分析了小麦幼穗在减数分裂期进行低温胁迫处理的基因表达特征。结果表明，在减数分裂期，低温胁迫可引起花药组织超微结构的损伤，导致籽粒结实率显著下降。幼穗在低温胁迫过程中的差异表达基因主要涉及激素信号转导、光合作用、昼夜节律-植物、糖代谢、DNA复制等反应通路。编码肌动蛋白解聚因子(ADF)、胚胎发育晚期丰富蛋白(LEA)、CCCH锌指蛋白等的基因在低温处理各个时间节点均极显著上调表达，可能参与调控小麦幼穗对低温胁迫的抗性反应。 |
| 英文摘要: |
| The late spring cold disaster caused by low temperature in spring occurs frequently in China. It is of great significance to analyze the gene regulation mechanism of wheat in response to low temperature to resist the genetic improvement. In this study, the gene expression characteristics of wheat young spikes subjected to low temperature during meiosis was analyzed using transcriptome technology under artificially controlled environmental conditions. The results indicate that low temperature during meiosis stage can cause damage to the ultrastructure of anther tissue, leading to a significant decrease in seed setting rate. The differentially expressed genes(DEGs) in young spikes under low temperature mainly involve pathways such as hormone signal transduction, photosynthesis, circadian rhythm-plants, sugar metabolism, DNA replication, etc. The encoding genes of actin depolymerization factor(ADF), late embryonic development abundant protein(LEA), CCCH zinc finger protein, etc. were significantly upregulated at all time points of low temperature treatment, which may be involved in regulating the resistance response of wheat spikes to low temperature. |
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