| 马 博，杜旭冉，武郅忠，郭荣娣，罗 爽，聂小军.野生二粒小麦ARF家族基因的鉴定和盐胁迫下的表达分析[J].麦类作物学报,2025,(11):1441 |
| 野生二粒小麦ARF家族基因的鉴定和盐胁迫下的表达分析 |
| Identification of Gene Family of ARF Transcription Factors in Wild Emmer Wheat and Analysis of Their Expression under Salt Stress |
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| DOI： |
| 中文关键词: 野生二粒小麦 ARF基因家族 遗传变异 盐胁迫 表达特性 |
| 英文关键词:Wild emmer ARF gene family Genetic variation Salt stress Expression profile |
| 基金项目:国家自然科学基金项目（U22A20457） |
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| 摘要点击次数: 92 |
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| 中文摘要: |
| 生长素响应因子(auxin response factor，ARF)是调控植物生长发育的关键转录因子之一，通过与生长素响应元件结合，参与生长素相关信号通路，在植物的器官建成、信号传导以及逆境响应等方面发挥着重要作用。为探究野生二粒小麦盐胁迫响应ARF基因及其与盐胁迫的关系，利用Blast和HMM-search相结合的方法在全基因组水平鉴定了野生二粒小麦的ARF家族基因,并对其启动子及耐盐性进行分析。结果表明，在野生二粒小麦中鉴定出22个ARF基因，其分布于2A、2B、3A、3B、5A、5B、6A、6B、7A和7B染色体上；利用系统进化树可将22个ARF家族成员分成3个亚家族。通过启动子顺式元件预测，野生二粒小麦ARF基因启动子主要包含与生长发育、激素响应、光响应及逆境响应相关4类顺式作用元件。经过遗传变异分析，在四倍体小麦的驯化过程中，ARF家族成员的遗传多样性降低，发生了明显的遗传瓶颈效益。在转录组数据中检测到17个成员响应盐胁迫，在盐胁迫下TdARF主要呈现下调表达。TdARF可为小麦耐盐育种的候选基因。 |
| 英文摘要: |
| Auxin response factor(ARF) is one of the key transcription factors that regulate plant growth and development. By binding to auxin response elements and participating in auxin related signaling pathways, ARF plays an important role in plant organ formation, signal transduction and stress response. To identify and mine ARF genes in response to salt stress in wild emmer wheat, we used a combination of Blast and HMM-search to identify ARF family genes at the genome-wide level in wild emmer wheat. The results showed twenty-two ARF genes were identified in wild emmer wheat, which are distributed on 2A, 2B, 3A, 3B, 5A, 5B, 6A, 6B, 7A, and 7B chromosomes. The 22 ARF family members were divided into three subgroups by phylogenetic tree. The prediction of promoter cis-elements showed that the promoter of ARF gene in wild emmer wheat mainly contain four types of cis-acting elements related to growth and development, hormone response, light response, and stress response. The analysis of genetic variation showed that the ARF family had a significant genetic bottleneck benefit with decreased genetic diversity during the domestication of emmer wheat. Seventeen TdARF were detected in the transcriptome data in response to salt stress. qPCR analysis showed that TdARF was mainly down-regulated under salt stress. It is suggested that TdARF may be used as candidate genes for wheat salt tolerance breeding. |
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