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| TaDGAT的克隆及其在花药发育中的潜在功能分析 |
| Cloning of TaDGAT and analysis of its potential function in anther development in wheat |
| 投稿时间：2025-10-31 修订日期：2026-01-15 |
| DOI： |
| 中文关键词: 小麦 光温敏雄性不育 DGAT 表达分析 荧光定量PCR |
| 英文关键词:wheat photothermal-sensitive male sterility DGAT expression analysis fluorescence quantitative PCR |
| 基金项目:北京市农林科学院院攻关项目(KJCX20251004)；北京市农林科学院探索项目(TSXM202520) |
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| 中文摘要: |
| 为探究二酰甘油酰基转移酶（diacylglycerol acyltransferase, DGAT） 在小麦光温敏雄性不育系花药发育中的潜在功能，本研究以小麦光温敏核雄性不育系BS366为材料，根据前期转录组测序结果筛选出TaDGAT（TraesCS1A02G125300），并在BS366中克隆获得拷贝序列，利用生物信息学分析预测TaDGAT基因及其编码蛋白质的结构与功能特征。结果表明，该基因开放阅读框（ORF）为1 542 bp，共编码513个氨基酸，根据蛋白质结构推测该蛋白为O-酰基转移酶。多序列比对与系统发育树分析显示该基因（蛋白）在小麦与大麦中亲缘关系最近。且发现miR398、miR169等miRNAs可能与TaDGAT互作参与脂代谢过程，进而调控花药发育；蛋白互作预测发现主要与O-酰基转移酶等脂质代谢途径中的关键蛋白互作。亚细胞定位预测TaDGAT位于细胞膜和高尔基体，参与生物膜的形成。基因表达模式显示，可育环境下花药中TaDGAT表达低，而在不育环境下的花药中则表达高。综上结果表明该基因可能通过miR398、miR169等miRNA及O-酰基转移酶等脂质代谢途径中的关键蛋白互作，从而参与花药发育（花粉形成）。该研究为解析DGAT基因在小麦中的功能提供一定的参考。 |
| 英文摘要: |
| To explore the potential function of diacylglycerol acyltransferase (DGAT) in wheat photo-thermo-sensitive genic male sterility (PTGMS) line, we focused on TaDGAT (TraesCS1A02G125300), screened out from previous transcriptome sequencing and cloned from PTGMS line BS366. Bioinformatics analyses revealed that the open reading frame (ORF) of this gene spans 1542 base pairs, encoding a total of 513 amino acids. Based on both secondary and tertiary structural analyses, it can be inferred that this protein functions as an O-acyltransferase. Through multiple sequence alignment and phylogenetic tree construction, it was found that wheat and barely have the closest genetic relationship. Additionally, it was found that microRNAs (miRNAs) such as miR398 and miR169 may interact with TaDGAT to participate in lipid metabolism, thereby regulating anther development. Protein-protein interaction prediction revealed that TaDGAT mainly interacts with key proteins in lipid metabolism pathways, including O-acyltransferases. Subcellular localization prediction indicated that TaDGAT is located in the cell membrane and Golgi apparatus, where it participates in the formation of biological membranes. The gene expression pattern showed that the expression level of TaDGAT in anthers was low under fertile conditions, while it was high under sterile conditions. In conclusion, the results indicated that the TaDGAT gene might participate in anther development (pollen formation) through interactions with miRNAs including miR398 and miR169, as well as key proteins in lipid metabolism pathways like O-acyltransferases. This study provides a certain reference for deciphering the function of the DGAT gene in wheat. |
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