|
| 普通小麦-野生二粒小麦远缘杂交后代穗发芽抗性鉴定和分子标记分析 |
| Identification and Molecular Marker Analysis of Pre-Harvest Sprouting Resistant of Common Wheat-Wild Emmer Distant Hybridsnt Hybrids |
| 投稿时间：2025-10-23 修订日期：2025-12-02 |
| DOI： |
| 中文关键词: 小麦 二粒小麦 远缘杂交 穗发芽 分子标记 |
| 英文关键词:Wheat Emmer wheat Distant hybridization Pre-harvest sprouting Molecular markers |
| 基金项目:国家自然科学基金(U1304320,U1904108)；河南省科技攻关项目(202102110022) |
|
| 摘要点击次数: 93 |
| 全文下载次数: 0 |
| 中文摘要: |
| 野生二粒小麦是普通小麦的近缘种，具有很多优良性状和抗性基因，将其与普通小麦杂交有助于培育创新种质，扩展小麦的遗传基础，提高小麦抗逆性。采用整穗发芽法（SGR）对野生二粒小麦与普通小麦杂交并回交获得的28份远缘杂交后代新品系进行了穗发芽抗性评价，并利用4个抗穗发芽功能性分子标记（PM19-A1、myb10D、Vp1B3和Dorm-B1）对试验材料进行基因型检测。结果表明,28份小麦后代品系相对穗发芽指数为0～1.09，平均相对穗发芽指数为0.38。其中达到高抗（HR）的品系有6份，占比21.43%；抗穗发芽材料有3份，占比10.71%；中抗穗发芽材料有9份，占比32.14%；感穗发芽材料有2份，占比7.14%；高感穗发芽材料有8份，占比28.57%。通过对抗穗发芽等位基因分析，等位变异组合PM19-A1a/VP-1Ba/myb10-D1b/Dorm-B1a的平均相对穗发芽指数最低,为0.13； PM19-A1b/VP-1Bb/myb10-D1b/Dorm-B1a的平均相对穗发芽指数最高，为0.70。综合表型鉴定和分子标记检测结果，筛选出MM-3-1、MM-3-5、MM-3-7、MM-3-0、MM-4(12-20)-2、MM-4-8这6份材料可作为抗穗发芽种质资源加以重点利用。 |
| 英文摘要: |
| Wild emmer wheat, a close relative of common wheat, possessing many excellent traits and resistance genes. Its hybridization with common wheat helps cultivate innovative germplasm, expand the genetic base of wheat, and enhance its stress resilience. Using the whole spike germination method (SGR), 28 new wheat lines derived from distant hybridisation and backcrossing between wild emmer wheat and common wheat were evaluated for spike germination resistance, and the experimental materials were genotyped using 4 functional molecular markers for pre-harvest sprouting resistance (PM19-A1、myb10D、Vp1B3 and Dorm-B1). The results showed that the relative spike germination index of the 28 wheat progeny lines ranged from 0 to 1.09, with an average relative spike germination index of 0.38. Among them, 6 lines reached high resistance (HR), accounting for 21.43%; 3 lines were resistant, accounting for 10.71%; 9 lines showed moderate resistance, accounting for 32.14%; 2 lines were susceptible, accounting for 7.14%; and 8 lines were highly susceptible, accounting for 28.57%. By analysing the alleles for pre-harvest sprouting resistance, the allelic combination PM19-A1a/VP-1Ba/myb10-D1b/Dorm-B1a had the lowest average relative spike germination index of 0.13, while PM19-A1b/VP-1Bb/myb10-D1b/Dorm-B1a had the highest average relative spike germination index of 0.70. Based on comprehensive phenotypic identification and molecular marker detection, 6 materials, namely MM-3-1, MM-3-5, MM-3-7, MM-3-0, MM-4 (12-20)-2 and MM-4-8, were selected for prioritizing utilisation as germplasm resources resistant to pre-harvest sprouting. |
|
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
基本信息
