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基金项目:财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-05-02A-02)；甘肃省现代农业产业技术体系(麦类作物)；甘肃省教育厅产业支撑计划项目(2021CYZC-12)；甘肃省陇原青年英才项目(2023)；甘肃农业大学伏羲青年英才计划(Gaufx-03Y06,Gaufx-04Y011)资助

作者	单位	地址
王长杰	甘肃农业大学农学院	甘肃省兰州市安宁区甘肃农业大学
汪欣瑶
王勇胜
牛丽
张宏
姚立蓉
司二静
杨轲
孟亚雄
马小乐
王化俊	甘肃农业大学农学院	甘肃省兰州市安宁区甘肃农业大学
汪军成^*	甘肃农业大学农学院	甘肃农业大学农学院

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中文摘要:

R2R3-MYB转录因子家族是MYB转录因子中最大亚家族，在植物多种胁迫响应中扮演重要角色，但其在低磷（P）胁迫下的研究鲜有报道。因此，本研究通过使用生物信息学方法，在大麦MorexV3基因组中鉴定出127个R2R3-MYB基因，并命名为Hv2RMYB-1至Hv2RMYB-127。这些基因主要分布于大麦3H染色体上，且启动子区富含激素响应顺式作用元件。理化性质分析表明，大多数成员编码酸性且不稳定的蛋白质。系统发育及共线性分析将大麦R2R3-MYB家族划分为5个不同的分支，16对串联复制基因。结合转录组数据（RNA-Seq）和实时荧光定量PCR技术（RT-qPCR），检测出10个大麦R2R3-MYB基因在低磷胁迫下均有不同程度的差异性表达，其中Hv2RMYB-48表达较为显著。进一步研究发现，Hv2RMYB-48定位于细胞核，其可以与MYB-ABRE-MYB顺式作用元件进行互作。这些结果为大麦R2R3-MYB转录因子在非生物胁迫响应中的功能提供了新见解，并为深入研究Hv2RMYB-48在低磷胁迫下的调控机制奠定了基础。

英文摘要:

The R2R3-MYB transcription factor family, the largest subfamily of MYB transcription factors, plays critical roles in various plant stress responses; however, its function under low-phosphate (P) stress remains poorly characterized. In this study, a total of 127 R2R3-MYB genes were identified in the barley MorexV3 genome using bioinformatic approaches and designated Hv2RMYB-1 to Hv2RMYB-127. These genes were predominantly distributed on chromosome 3H and were found to harbor hormone-responsive cis-acting elements in their promoter regions. Physicochemical analysis revealed that most members encode acidic and unstable proteins. Phylogenetic and collinearity analyses classified the barley R2R3-MYB family into five distinct clades, including 16 pairs of tandemly duplicated genes. Based on transcriptomic data (RNA-Seq) and quantitative real-time PCR (RT-qPCR), ten barley R2R3-MYB genes showed differential expression under low-P stress, among which Hv2RMYB-48 was notably upregulated. Further investigation confirmed that Hv2RMYB-48 localizes to the nucleus and can bind to the MYB-ABRE-MYB cis-element. These findings provide new insights into the role of barley R2R3-MYB transcription factors in abiotic stress responses and lay a foundation for further elucidating the regulatory mechanism of Hv2RMYB-48 under low-phosphate conditions.

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