| To investigate the effects of sulfur fertilization on wheat yield and quality under different nitrogen levels in the lime concretion black soil area of northern Anhui Province, a field split plot experiment was conducted in Suzhou, Anhui Province, from 2022 to 2024. The wheat varieties tested were Annongda 1216 and Huachengmai 1688. The main plots were based on a basal nitrogen application of 196 kg·hm-2, with two top dressed nitrogen levels of 0 kg·hm-2 (N1) and 75 kg·hm-2 (N2) at the jointing stage. The sub plot was set up with two sulfur levels of 0 kg·hm-2(S1) and 18.29 kg·hm-2 (S2). The study compared and analyzed the effects of different treatments on wheat yield, quality, dry matter accumulation and translocation, and photosynthetic characteristics. The results showed that compared to the N1S1 treatment, the N2S2 treatment increased the average values of leaf area index(LAI), spike number, grain number per spike, grain yield, and protein content by 13.43%, 6.29%, 2.82%, 12.17%, and 1.07 percentage points, respectively. Under the same nitrogen application rate, Pn, Gs, and Tr in the S2 treatment were higher than those in S1, while Ci was lower. The post flowering dry matter accumulation and grain dry matter distribution at maturity stage were on average 7.78% and 5.60% higher in S2, and the spike number, grain number per spike, and yield increased by 3.75%, 1.70%, and 6.14%, respectively. Under the same nitrogen sulfur fertilization level, N2 resulted in higher tiller number and leaf area index than N1, with Pn, Gs, and Tr increasing by 5.67%, 16.62%, and 12.41%, respectively. The post flowering dry matter accumulation and grain dry matter distribution at maturity stage were on average 8.47% and 6.23% higher, and the spike number, grain number per spike, yield, and protein content increased by 3.16%, 1.39%, 6.23%, and 2.05 percentage points, respectively. In conclusion, top dressing nitrogen fertilizer and basal sulfur application can enhance wheat leaf area index, improve photosynthetic capacity, promote post flowering dry matter accumulation, and ultimately increase wheat grain yield and protein content. |