欢迎访问《麦类作物学报》编辑部网站

作者	单位
李婷婷, 周雪, 柴永懋,王中华	（1.陕西农林职业技术大学，陕西杨凌 712100； 2.延安市农业科学研究院,陕西延安716000； 3.西北农林科技大学农学院,陕西杨凌 712100）

摘要点击次数: 72

全文下载次数: 42

中文摘要:

为探究细胞分裂素对小麦根系形态建成的调控作用，并挖掘调控根系发育的关键功能基因，系统分析了0.05 μmol·L^-1 6-BA处理后小麦品种晋麦47幼苗根系表型的变化，评价了110份小麦品种（系）对6-BA的敏感性，同时采用同源克隆方法获得细胞分裂素氧化酶/脱氢酶基因TaCKX1B，并通过烟草异源过表达验证其功能。结果表明，0.05 μmol·L^-1 6-BA处理显著抑制小麦初生根伸长生长，促进根毛发生与伸长；小麦对6-BA敏感性存在基因型差异，敏感指数主要分布于40%~60%，且苗期低敏感材料在灌浆期表现出更发达的根系表型；TaCKX1B基因定位于1B染色体，编码区全长1 569 bp，烟草过表达该基因后T₁代转基因植株根系生物量约为野生型的2倍。综上所述，TaCKX1B基因在正向调控植物根系发育中具有重要作用。

英文摘要:

This study aimed to investigate the regulatory role of cell division cytokinin in wheat root morphological development and to identify key functional genes involved in root system architecture regulation. The seedlings of a wheat variety Jinmai 47 were treated with exogenous 0.05 μmol·L^-1 6-benzylaminopurine(6-BA) to systematically analyze its effects on root phenotypic traits. The genotypic sensitivity of 110 wheat varieties(lines) to 6-BA was evaluated. Furthermore, the TaCKX1B gene was obtained through homology-based cloning, and its function was validated via heterologous overexpression in tobacco. The results showed that the treatment with 0.05 μmol·L-1 6-BA significantly inhibited primary root elongation while promoting root hair initiation and elongation. Considerable genetic variation in sensitivity to 6-BA was observed among wheat genotypes, with sensitivity indices predominantly ranging from 40% to 60%. Materials with low sensitivity at the seedling stage exhibited more developed root systems at the grain-filling stage. The TaCKX1B gene was located on chromosome 1B, with a coding sequence length of 1 569 bp. Heterologous overexpression of TaCKX1B in tobacco resulted in approximately a two-fold increase in root biomass in T₁ transgenic plants compared to wild-type controls. In conclusion, the TaCKX1B gene plays a positive regulatory role in plant root development.

查看全文查看/发表评论下载PDF阅读器

关闭