| Somatic embryogenesis receptor-like kinases(SERK) can sense and transmit extracellular signals which located on the cell membrane, play an important role in plant growth, development, and stress response. We identified SERKs in barley in order to explore the expression pattern of HvSERKs under drought and salt stress. We used bioinformatics methods to identify and analyze the HvSERKs from whole genome of barley with SERKs sequence of Arabidopsis as reference sequence, and analyzed their expression under drought and salt stress by qRT-PCR. The results in this article showed that eight HvSERKs locate in the barley genome, three of which were on 7H chromosome. The phylogenetic tree constructed with SERKs in Arabidopsis, wheat, rice, maize, rape, potato and barley showed that HvSERK1, HvSERK3, HvSERK6 and HvSERK8 of barley formed a separate branch. Gene structure and conserved protein motif analysis showed that HvSERKs were highly similar and conserved. The HvSERKs were located in the plasma membrane except for HvSERK7 which in the chloroplast, and secondary structures were mainly random coils. Under drought and salt condition, the expression level of HvSERKs except for HvSERK4 which is down-regulated in leaves with salt treatment is up-regulated. Additionally, HvSERKs genes are highly expressed in mature embryos compared to immature embryos. This study identified and analyzed the HvSERKs gene family in barley, and found that they respond to drought and salt stress, with the development regulation of embryos and spikes. |
基本信息
