| 王建飞，郭利建，高维东，胡城祯，车 卓，陈 涛，张沛沛，杨德龙.小麦FLA基因家族鉴定及粒重相关基因单倍型分析[J].麦类作物学报,2025,(10):1293 |
| 小麦FLA基因家族鉴定及粒重相关基因单倍型分析 |
| Genome-Wide Identification of FLA Gene Family and Haplotype Analysis of Kernel Weight-Related Genes in Wheat(Triticum aestivum L.) |
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| DOI： |
| 中文关键词: 小麦 FLA基因家族 表达模式 单倍型分析 SNP标记 千粒重 |
| 英文关键词:Wheat FLA gene family Gene expression Haplotype analysis SNP marker Thousand-kernel weight |
| 基金项目:甘肃省农业农村厅种业攻关项目（GYGG-2024-2）；甘肃省科技重大专项（22ZD6NA009）；甘肃省科技厅基础研究创新群体项目（24JRRA633）；国家自然科学基金项目（32360518,32260520,32160487）；甘肃省高校科研创新平台重点培育项目（2024CXPT-01）；中央引导地方科技发展资金项目（23ZYQA0322）；甘肃省高等学校产业支撑计划项目（2022CYZC-44）；甘肃省教育厅青年博士支持项目（2024QB-062）；省部共建干旱生境作物学国家重点实验室开放基金项目（GSCS-2023-07）；甘肃农业大学博士科研启动项目（GAU-KYQD-2020-6）；甘肃省教育厅2025年研究生创新之星项目（2025CXZX-853） |
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| 中文摘要: |
| 类成束阿拉伯半乳糖蛋白（fasciclin-like arabinogalactan-protein, FLA）是一类高度糖基化的蛋白，广泛参与植物生长发育及抗逆境胁迫等途径，但其在小麦中的作用机制尚不清楚。为了解FLA在小麦中的分布及其生物学功能，利用生物信息学手段在全基因组水平鉴定了小麦FLA家族成员并对其进行了系统分析，利用RNA-Seq及qRT-PCR检测TaFLAs基因在不同组织和逆境胁迫中的表达模式，通过单倍型分析候选基因与粒重相关性状的关系。结果表明，在小麦中共鉴定到71个TaFLAs基因，分布在17条染色体上，依次命名为TaFLA1~TaFLA71。71个基因按照亲缘关系被聚类为8个亚族，同一亚族的不同成员间具有相似的基因结构和保守基序，推测其具有相似的功能。通过种内同线性分析，71个TaFLAs基因存在85个片段复制基因对，其中30对为基因间重复，55对为基因内重复；通过种间共线性分析，TaFLAs基因与二穗短柄草的BdFLAs亲缘关系较近。经RNA-Seq及qRT-PCR分析，TaFLAs在不同组织、非生物胁迫中普遍表达，其中TaFLA15、TaFLA34和TaFLA62在穗和籽粒中表达量较高，TaFLA5、TaFLA15、TaFLA34和TaFLA62在PEG 6000处理下上调表达，TaFLA5和TaFLA34在NaCl处理下上调表达。针对TaFLA62开发了SNP分子标记，通过结合地理时空分布进行关联分析，TaFLA62-HapⅡ是增加粒重的优异单倍型，且在小麦育种过程中受到正向选择。综合来看，小麦TaFLAs的基因结构相似，大多数能够响应逆境胁迫，其TaFLA62-HapⅡ单倍体型可用于小麦产量性状的改良。 |
| 英文摘要: |
| Abstract:Fasciclin-like arabinogalactan protein(FLA), a class of highly glycosylated proteins, are widely involved in plant growth, development and stress responses, yet their functional mechanisms in wheat remain unclear. To investigate the members and biological roles of FLA in wheat, this study conducted genome-wide identification and systematic analysis of the wheat FLA family using bioinformatics approaches. The RNA-Seq and qRT-PCR were employed to examine the expression patterns of TaFLAs across tissues and under abiotic stresses, while haplotype analysis was performed to explore the association between candidate genes and kernel weight-related traits. A total of 71 TaFLAs were identified in wheat, distributed across 17 chromosomes and designated TaFLA1 to TaFLA71. Phylogenetic analysis classified these genes into eight subfamilies, with members within the same subfamily exhibiting conserved gene structures and motifs, suggesting functional similarity. Intraspecific synteny analysis revealed 85 segmental duplication pairs(30 interchromosomal and 55 intrachromosomal duplications), while interspecific synteny highlighted closer evolutionary relationships between TaFLAs and BdFLAs from Brachypodium distachyon. The RNA-Seq and qRT-PCR results demonstrated widespread expression of TaFLAs across tissues and under stress conditions. Notably, TaFLA15, TaFLA34 and TaFLA62 showed high expression in spikes and grains, while TaFLA5, TaFLA15, TaFLA34 and TaFLA62 were upregulated under PEG 6000 treatment; TaFLA5 and TaFLA34 were induced by NaCl stress. A SNP molecular marker developed for TaFLA62 revealed, combined association analysis and geographical-temporal distribution studies, that the TaFLA62-HapⅡ haplotype significantly enhances kernel weight and has undergone positive selection during wheat breeding. Overall, the gene structure of wheat TaFLAs is similar, and most of them can respond to adversity stress, and its TaFLA62-apⅡ haplotype can be used to improve wheat yield traits. |
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