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了解不同1BL.1RS易位品种中的1RS结构差异,有利于该易位染色体在小麦育种中的应用。以小麦品种南麦618为亲本,分别与矮抗58、川农17、兰考矮早8、陕354、烟农18、晋麦45等13个1BL.1RS易位小麦品种和1个7DL.1RS易位品种鲁麦8号杂交。基于串联重复序列pSc119.2、pSc200和pSc250,设计了GC碱基含量高的寡核苷酸探针200G、250G、Oligo-119.2-2和AT碱基含量高的寡核苷酸探针200T、250T、Oligo-119.2-3。利用这些探针对14个杂交组合的F1植株根尖中期染色体进行ND-FISH分析。除Oligo-119.2-3不能产生信号外,其余5个探针都在不同来源的1RS上产生了强弱不同的信号,体现了1RS的结构差异,由此将15份材料中的1RS分为4个类型。探针200G、200T和Oligo-119.2-2在1RS中体现出一致的变异,而250G和250T体现的变异不一致,表明不同1RS的pSc250家族串联重复序列的GC和AT碱基含量存在差异。本研究结果表明用于小麦品种中的1RS存在结构多样性,为进一步追溯这些1RS的来源提供了参考。

英文摘要:

Understanding the 1RS structural variations in different 1BL.1RS translocation cultivars is favorable for the application of this translocation in wheat breeding. The 1BL.1RS translocation cultivar Nanmai 618 crossed with 7DL.1RS translocation cultivar Lumai 8 and with 13 1BL.1RS translocation wheat cultivars, including Aikang 58, Chuannong 17, Lankaoaizao 8, Shaan 354, Yannong 18 and Jinmai 45, etc. Based on the tandem repeats pSc119.2, pSc200 and pSc250, oligonucleotide probes 200G, 250G and Oligo-119.2-2 with high GC content, and 200T, 250T and Oligo-119.2-3 with high AT content were designed. ND-FISH analysis of root tip metaphase chromosomes of the F1 plants from the 14 cross combinations was performed using these probes. Except for Oligo-119.2-3, which failed to produce a signal, the remaining five probes produced signals of different strengths on the 1RS, reflecting the structural differences of the 1RS, which led to the classification of the 1RS in the 15 materials into four types. Probes 200G, 200T, and Oligo-119.2-2 reflected consistent variation among the 1RS, whereas 250G and 250T reflected inconsistent variation, suggesting that the pSc250 family tandem repeats of different 1RS arms differ in GC and AT base compositionsdesed . The results of this study indicate the existence of structural diversity in 1RS arms used in wheat cultivars and provide a reference for further tracing the origin of these 1RS arms.

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