| In order to screen salt-tolerant germplasm of oat, 200 oat germplasm resources were subjected to double salt stress(NaCl∶Na2SO4∶NaHCO3∶ Na2CO3 = 1∶9∶9∶1 ). Six germination indexes such as seed vigor, bud length and root length were measured. The salt-tolerant ability of oat was comprehensively evaluated by principal component analysis( PCA ) and cluster analysis. The results showed that the combined saline-alkali stress significantly inhibited the germination and early growth of oat germplasm. The germination rate, germination potential, root length, bud length and seed vigor all decreased to varying degrees, among which the vigor index decreased the most, while the salt damage rate increased significantly. Two principal components were extracted by principal component analysis, and the cumulative variance contribution rate was 81.571 %. Principal component 1 exhibited a higher loading on seed vigor, shoot length, root length, and germination potential, reflecting traits related to the germination potential of oats. Principal component 2 was mainly associated with salt damage rate and shoot length, reflecting the tolerance performance of seeds under salt-alkali stress. Based on the D-value clustering analysis, the germplasms were classified into five groups: highly salt-alkali tolerant group (D≥0.850, 2 germplasms), salt-alkali tolerant group (0.850 > D≥0.720, 2 germplasms), moderately salt-alkali tolerant group (0.720 > D≥0.460, 15 germplasms), salt-alkali sensitive group (0.460 > D≥0.305, 58 germplasms), and extremely salt-alkali sensitive group (D < 0.305, 123 germplasms). Two oat germplasm lines with high tolerance ( 163,21 ) and two oat germplasm lines with tolerance ( B106, B60 ) were screened. |
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