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作者	单位
顾家依，吴婉婷，蒲天宇，徐继伟，章岩，朱昌进，黄涵语，李国辉，许轲	（江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室，江苏省粮食作物现代产业技术协同创新中心，扬州大学，江苏扬州 225009）

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中文摘要:

为探讨纳米碳点对小麦出苗、苗期形态和生理特征及产量的影响，以小麦品种扬麦25号为材料，设置纳米碳点水溶液浸种、拌种、出苗后叶面喷施三种施用方式，以及0.02、0.1和0.5 mg·mL^-1三种碳点浓度，同时以不施用纳米碳点处理作为对照(CK)，分析了不同处理下小麦出苗率、株高、生物量、抗逆生理及产量的差异。结果表明，碳点水溶液浸种和拌种后小麦田间实际出苗率（播后20 d）分别达到76.8%~78.0%和58.4%~60.5%，较CK分别增加了27.0~28.2和8.6~10.7个百分点。与CK相比，纳米碳点浸种、拌种和叶面喷施处理下小麦幼苗（播后20 d）的株高分别增加了39.8%~52.5%、25.6%~28.1%和23.3%~32.2%，地上部鲜重分别增加95.3%~100.4%、46.9%~53.8%和45.3%~56.5%，最大根长分别增加58.4%~60.7%、28.6%~35.4%和19.2%~34.7%，根鲜重分别增加60.2%~85.8%、45.5%~48.1%和36.2%~39.1%；同时，纳米碳点浸种、拌种和叶面喷施后小麦幼苗α-淀粉酶、β-淀粉酶和抗氧化酶活性及可溶性糖含量均显著提高，丙二醛含量降低，产量分别增加了13.8%~16.0%、9.8%~10.6%和5.4%~7.0%。上述结果说明，利用纳米碳点浸种、拌种和叶面喷施能有效促进小麦出苗和早发壮苗，增强其抗逆性，提高产量，其中以0.02~0.5 mg·mL^-1纳米碳点溶液浸种的效果最佳。

英文摘要:

To investigate the effects of nano-carbon dots on wheat emergence, the morphological and physiological characteristics of seedling and yield, a total of three treatments were set up: seed soaking, seed dressing, foliar spraying after emergence with nano-carbon dots aqueous solution, and no application of nano-carbon dots was used as the control(CK), with a wheat variety Yangmai 25 as the material, Three carbon dots concentrations were applied for seed soaking, seed dressing, and foliar spraying, with concentrations of 0.02, 0.1, and 0.5 mg·mL^-1, respectively. The differences in wheat emergence rate, plant height, biomass, stress resistance physiology, and yield under different treatments were analyzed. The results showed that using carbon dots aqueous solution for seed soaking and seed dressing, the actual emergence rates in the field after 20 days of sowing reached 76.8%-78.0% and 58.4%-60.5%, respectively. Compared with CK, the emergence rates of wheat in the field were increased by 27.0-28.2 percentage points and 8.6-10.7 percentage points, respectively. Compared with CK, after 20 days of wheat sowing, the height of seedlings treated with seed soaking, seed dressing, and foliar spraying increased by 39.8%-52.5%, 25.6%-28.1%, and 23.3%-32.2%, respectively; the fresh weight of aboveground parts increased by 95.3%-100.4%, 46.9%-53.8%, and 45.3%-56.5%, respectively; the maximum root length increased by 58.4%-60.7%, 28.6%-35.4%, and 19.2%-34.7%, and the fresh weight of roots increased by 60.2%-85.8%, 45.5%-48.1%, and 36.2%-39.1%, respectively. After seed soaking, seed dressing, and foliar spraying with nano-carbon dots, wheat seedling’ α-amylase and β-amylase activities, antioxidant enzyme activity, and content of soluble sugar were significantly increased, and the content of malondialdehyde was decreased. The wheat yield increased by 13.8%-16.0%, 9.8%-10.6%, and 5.4%-7.0%, respectively. The above results indicated that using nano-carbon dots for seed soaking, seed dressing, and foliar spraying led to the effectively promoted wheat emergence, early emergence and strong seedling, enhanced its stress resistance, and thus increased yield, and using 0.02-0.5 mg·mL^-1 nano-carbon dots solution to soak seeds can achieve the best effect in wheat production.

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