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作者	单位
董飞，张贤，张敏敏，贾亚琴，申艳婷，李峰，闫双堆，闫秋艳	（1.山西农业大学小麦研究所，山西临汾 041000； 2.临汾职业技术学院，山西临汾 041000； 3.山西农业大学资源环境学院，山西太谷 030801）

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中文摘要:

为探讨不同粒色小麦在不同肥力土壤下籽粒抗氧化能力差异，选取5个黑粒、2个蓝粒及1个白粒小麦品种（系）为材料，设置高、低两个肥力土壤，分析小麦籽粒花色苷、总酚及类黄酮的含量，并通过测定铁离子还原能力（FRAP值）、1,1-二苯基-2-三硝基苯肼（DPPH·）自由基清除率、2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS⁺·）自由基清除率以及羟基（·OH）自由基清除率等评价籽粒抗氧化能力。结果表明，低肥力土壤有利于籽粒花色苷积累，其含量较高肥力土壤增加33.20%~73.57%，其中蓝粒小麦含量较高，黑粒小麦次之，白粒小麦最低；籽粒总酚和黄酮含量较高肥力土壤分别下降4.12%~56.46%和1.14%~25.87%；肥力、品种、肥力×品种对花色苷、总酚和黄酮含量均具有显著影响。高肥力土壤籽粒FRAP值和ABTS⁺·清除率均高于低肥力土壤，而DPPH·和·OH清除率总体表现为低肥力土壤高于高肥力土壤；土壤全氮和有机质含量是抗氧化能力的主要调控因子；肥力、品种、肥力×品种对FRAP值及ABTS⁺·、DPPH·和·OH清除率均具有显著影响。高低肥力土壤综合得分大小均表现为黑粒小麦＞蓝粒小麦＞白粒小麦；高肥力土壤平均得分高于低肥力土壤。综上，高肥力土壤较低肥力土壤种植的小麦具有较高的抗氧化能力，其中黑粒小麦的抗氧化能力高于蓝粒和白粒小麦。

英文摘要:

In order to investigate the differences in antioxidant capacity of wheat grains with different grain colors under high and low soil fertility conditions, five black-grained wheat, two blue-grained wheat and one white-grained wheat variety(lines) were selected to determine the contents of anthocyanins, total phenols and flavonoids in grains. The antioxidant capacity of the grains was evaluated by measuring the ferricion reducing antioxidant potential(FRAP value), 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH·) radical scavenging rate, 2, 2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS⁺·) radical scavenging rate, and hydroxyl(·OH) radical scavenging rate. The results showed that low fertility soil was conducive to the accumulation of anthocyanins in grains, and the anthocyanin content were increased by 33.20%-73.57% compared to those in high fertility soil. Among them, blue-grained wheat had a higher content, followed by black-grained wheat, and white-grained wheat had the lowest content; The content of total phenols and flavonoids in grains was decreased by 4.12%-56.46% and 1.14%-25.87%, respectively. Fertility, variety, and fertility×variety had significant effects on the content of anthocyanins, total phenols, and flavonoids. The FRAP value and ABTS⁺· clearance rate of grains in high fertility soils were higher than those in low fertility soils, while the overall DPPH and ·OH clearance rates were higher in low fertility soils than in high fertility soils. The total nitrogen and organic matter content in soil were the main regulatory factors of antioxidant capacity. Fertility, variety, and fertility×variety had significant effects on FRAP values and ABTS⁺·, DPPH, and ·OH clearance rates. The comprehensive score of high and low fertility soil ranked as black-grained wheat>blue-grained wheat>white-grained wheat. The average score of high fertility soil was higher than that of low fertility soil. In summary, wheat grown in high fertility soil has higher antioxidant capacity than that in low fertility soil, with black-grained wheat was higher than blue- and white-grained wheat.

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