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作者	单位
董岩，李硕，缪倩，靳道然，杨荣明，季英华	（1.江苏省农业科学院植物保护研究所，江苏南京 210014； 2.江苏省植物保护植物检疫站，江苏南京 210014）

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中文摘要:

在对江苏小麦病害进行调查时发现疑似感染大麦黄矮病毒（barley yellow dwarf viruses, BYDVs）的病样，病株旗叶由叶尖向叶基扩展变黄，黄化面积占全叶的1/3~1/2。为明确其感染的病毒种类，对田间采集的样品进行检测和鉴定。结果表明，经利用BYDVs通用引物进行检测，采集江苏省8个地点的261份样品中有182份样品扩增到了目的条带。通过序列测定，8个地点样品中的病毒种类均为BYDV-PAV。利用BYDV-PAV外壳蛋白基因（CP）的特异引物对阳性样品进行检测，结果全部为阳性；经对克隆的CP基因序列聚类分析，江苏8个地点的BYDV与韩国的BYDV-PAV株系相对近缘。这是首次发现江苏小麦上的大麦黄矮病毒为PAV株系。

英文摘要:

An investigation on wheat disease in Jiangsu Province was carried out and the samples showed that the tip of the flag leaf expanded to the leaf base and became yellow, and the yellowing accounted for about 1/3 to 1/2 of the whole leaf, which was very similar with the symptoms induced by barley yellow dwarf viruses(BYDV). In order to detect and identify the possible virus associated with the disease, the samples were collected from eight locations in Jiangsu province. A total of 261 samples were detected with various wheat viruses using RT-PCR and 182 samples were found positive when a pair of degenerate primers for BYDV was used. The result of sequence analysis revealed that the virus shared the highest sequence identities with BYDV-PAV. The positive samples were further detected using specific primers for BYDV-PAV coat protein gene(CP) and the results showed that all samples were positive. Further phylogenetic analysis based on the sequence of BYDV-PAV CP gene revealed that the virus detected shared the highest sequence identities with the isolates from Korea. This is the first report of barley yellow dwarf virus-PAV in Wheat grown in Jiangsu province, China.

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