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作者	单位
石明玉，冯浩，李柱刚	（1.黑龙江大学生命科学学院/农业微生物技术教育部工程研究中心/黑龙江省寒区植物基因与生物发酵重点实验室，黑龙江哈尔滨 150080； 2.北京市农林科学院生物技术研究所，北京 100097）

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中文摘要:

热激转录因子（heat shock transcription factor, HSF）家族与植物生长发育、胁迫响应密切相关。为了解大麦HSF家族成员的功能，本研究利用生物信息学方法在大麦全基因组水平进行HSF家族成员鉴定，并对其理化性质、亚细胞定位、进化关系和共线性关系及基因结构、启动子顺式作用元件、蛋白互作网络进行了分析。结果发现，在大麦中共鉴定到的29个HSF基因，HvHSF蛋白的分子量介于10.24~56.59 kDa之间，属于稳定性较差的亲水蛋白，均预测定位于细胞核。进化树将大麦HvHSF基因分为A、B、C三类；共线性分析显示，HvHSF5d和HvHSF7b为共线基因对。结构域和基因结构分析发现，亲缘关系较近的HvHSF具有相似的结构域和基因结构。启动子顺式作用元件分析发现，HvHSF启动子含有多种与生长发育以及抗逆相关的顺式作用元件。蛋白互作网络预测表明，HvHSF可能参与植物胁迫响应调节和H₂O₂的清除。通过大麦地下部盐、碱胁迫转录组测序结合qRT-PCR验证发现，HvHSF在盐、碱胁迫下表达存在差异。盐、碱胁迫下，HvHSF1a、HvHSF1b、HvHSF2a、HvHSF3c、HvHSF5b表达量均有所增加；HvHSF6在盐胁迫下表达量增加，碱胁迫下降低。综上，HSF家族成员可能参与大麦耐盐、碱调控。

英文摘要:

The heat shock transcription factor(HSF) family is closely associated with plant growth, development and stress responses. To explore the function of the barley HSF gene family, this study used bioinformatics methods to identify HvHSF genes at the whole genome level and analyze the physicochemical properties, subcellular localization, evolutionary relationships, and collinearity relationships of the HvHSF proteins, as well as the gene structure, promoter cis-acting elements, and protein interaction networks of HvHSF. The results showed that the molecular weight of 29 HvHSF proteins ranges from 10.24 kDa to 56.59 kDa, classified as relatively unstable hydrophilic proteins, with a predicted localization in the nucleus. Evolutionary analysis showed that barley HvHSF genes can be divided into A, B and C groups and are more closely related to rice. Collinearity analysis revealed that HvHSF5d and HvHSF7b are collinear gene pairs. Domain and gene structure analyses revealed that closely related HvHSF genes have similar domain structures and gene structures. Promoter cis-acting element analysis showed that the HvHSF promoter contains various cis-acting elements related to growth and development, as well as stress responses. Protein interaction network predictions indicated that HvHSF may be involved in plant stress response regulation and H₂O₂ scavenging. Through analysis of the barley stress transcriptome combined with qRT-PCR validation, it was found that HvHSF exhibited differential expression under salt and alkali stresses. The expression levels of HvHSF1a, HvHSF1b, HvHSF2a, HvHSF3c and HvHSF5b were increased under salt and alkali stresses. However, HvHSF6 performed increased expression level under salt stress, but decreased expression level under alkali stress. This study confirmed that the HSF gene family responds to salt and alkali stresses and may contribute to the regulation of salt-alkali tolerance.

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