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作者	单位
冯政凯，杨晓莹，徐晓敏，黄晨曦，宋思源，丁旭，朱李亚，熊辉，陈春环，赵继新，王长有，李停栋，邓平川，吉万全	（西北农林科技大学农学院，陕西杨凌 712100）

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中文摘要:

淀粉分支酶是参与植株淀粉生物合成和代谢的关键酶之一。为了探究野生二粒小麦淀粉分支酶的功能，利用野生二粒小麦基因组数据，鉴定到9个淀粉分支酶(SBE) 基因，并对其理化性质、系统发育、顺式作用元件、共线性和表达模式进行了系统分析。结果表明，9个TdSBEs基因编码的蛋白分子量介于65 518.04~151 934.40 Da，等电点范围为5.37~6.64，亚细胞均定位于叶绿体。经系统进化分析，9个TdSBEs基因可划分为3个亚家族（TdSBEⅠ、TdSBEⅡ和TdSBEⅢ），同一亚家族内不同成员间具有高度相似的基因结构和motif组成。通过顺式作用元件分析，TdSBEs基因包含植物激素、植物生长发育和非生物胁迫相关的元件，同时在TdSBE2亚家族中鉴定到与胚乳和根特异性元件。经染色体定位和共线性分析，TdSBEs家族成员主要在第二和第七同源群，并鉴定到一对串联复制基因。经表达特性分析，TdSBEs基因主要在穗和籽粒中高表达，且其表达具有时空特异性。以上结果说明，野生二粒小麦中存在多个TdSBEs基因，且主要在穗和籽粒中表达，在启动子区域有大量与植物生长发育、植物激素以及逆境反应相关的顺式作用元件。

英文摘要:

Starch branching enzyme is one of the key enzymes involved in plant starch biosynthesis and metabolism. In order to explore the function of starch branching enzyme in wild emmer wheat, nine starch branching enzyme genes(TdSBEs) were identified based on the genome data of wild emmer wheat by bioinformatics methods, and their physical and chemical properties, phylogeny, cis-acting elements, collinearity, and expression pattern were analyzed. The results showed that the molecular weight of the protein for the nine TdSBEs was between 65 518.04 and 151 934.4 Da, with isoelectric point ranging from 5.37 to 6.64. The subcellular localization for all TdSBEs were in the chloroplast. Phylogenetic analysis showed that the nine TdSBEs were classified into 3 subfamilies. Majority of the gene within the same subfamily were conserved in gene structure and motif, except for TdSBE1-7A1. The TdSBEs contained the cis-elements related to plant hormones, plant growth and development, and abiotic stress response. In addition, endosperm and root specific-expression elements were also found in TdSBE2s. Chromosomal localization and collinearity analysis showed that the members of the TdSBEs were located in the group-2 and-7 homologous chromosomes, and a pair of tandem replication genes were identified. The TdSBEs were highly expressed in spike and grain, showing spatial-temporally specific expression. The above results indicate that there are multiple TdSBEs genes in wild emmer wheat, which are mainly expressed in spikes and grains. There are a large number of cis-acting elements related to plant growth and development, plant hormones, and stress response in the promoter region.

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