| 孙成群，张萌娜，洪 益，张宇航，李璐飞，许如根，朱 娟.大麦PYL、PP2CA和SnRK2家族基因鉴定及盐胁迫下的表达分析[J].麦类作物学报,2025,(6):735 |
| 大麦PYL、PP2CA和SnRK2家族基因鉴定及盐胁迫下的表达分析 |
| Identification of PYL, PP2CA and SnRK2 Family Genes in Barley(Hordeum vulgare L.) and Expression Analysis under Salt Stress |
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| DOI： |
| 中文关键词: 大麦 PYL PP2CA SnRK2 生物信息学 盐胁迫 |
| 英文关键词:Barley PYL PP2CA SnRK2 Bioinformatics Expression pattern |
| 基金项目:国家自然科学基金项目(32301871)；国家重点研发计划项目(2022YFD2301302， 2022YFD2301300)；国家大麦青稞产业技术体系专项(CARS-05)；江苏高校优势学科建设工程项目；2022年农业重大技术协同推广计划项目(2022-ZYXT-04-2) |
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| 中文摘要: |
| 脱落酸(abscisic acid, ABA)在植物生长、发育、衰老及胁迫响应等方面均发挥重要作用，其核心信号转导通路由PYL-PP2CA-SnRK2组成。为探究大麦PYL、PP2CA和SnRK2蛋白功能特征、表达模式及进化关系，本研究利用生物信息学方法对大麦PYL、PP2CA和SnRK2家族成员进行了全基因组鉴定，并分析其成员的基因结构、染色体定位、蛋白理化性质、启动子区顺式作用元件、组织表达以及逆境胁迫下的转录组表达量等。结果在大麦中共鉴定到10个HvPYL、13个HvPP2CA及9个HvSnRK2基因。HvPYLs和HvPP2CAs分布在1H~5H和7H，HvSnRK2s分布在1H~5H；HvPYLs和HvPP2CAs主要定位叶绿体，HvSnRK2s主要定位在细胞质中。三个家族成员的motif均具有高度的保守性，氨基酸数均小于500，启动子上含有大量响应激素元件。三个家族的基因在根、茎、花序和种子中表达量存在显著差异，HvPYL2在根部表达量最高，HvPYL9在种皮表达量最高；HvPP2C-A1~10在花序表达量较高；HvSnRK2.5、HvSnRK2.7、HvSnRK2.8在种皮表达量较高。对一对耐盐性差异显著的近等基因系在盐胁迫下48 h和60 h的转录组数据分析，结果显示，HvPP2C-A2、HvPP2C-A4、HvPP2C-A6、HvPP2C-A9、HvPP2C-A10和HvSnRK2.4受盐胁迫诱导显著上调表达，而HvPYL6和HvPYL7显著下调表达。实时荧光定量PCR验证结果与转录组数据一致。综合来看，PYL，PP2CA和SnRK2家族基因在大麦响应盐胁迫过程中扮演着重要角色。 |
| 英文摘要: |
| Abscisic acid(ABA) plays a crucial role in plant growth, development, senescence and stress response. The core signal transduction pathway is composed of PYL-PP2CA-SnRK2. In order to explore the protein functional characteristics, expression patterns and evolutionary relationships of PYL, PP2CA and SnRK2 in barley, the HvPYL, HvPP2CA and HvSnRK2 was identified from whole genome by bioinformatics methods. Gene structure and chromosomal localization, protein physicochemical properties, cis-acting elements in promoter region, expression analysis of different tissues and transcriptome expression patterns under salt stress were systematically analyzed. The results showed that 10 HvPYL, 13 HvPP2CA and 9 HvSnRK2 genes were identified from the barley genome. HvPYLs and HvPP2CAs were distributed in 1H-5H and 7H chromosomes, and HvSnRK2s were distributed in 1H-5H chromosomes. HvPYLs and HvPP2CAs were mainly located in chloroplasts, and HvSnRK2s are mainly localized in the cytoplasm. The motifs of the three family genes were highly conserved. The number of amino acids were less than 500, and the promoters contained a large number of plant response hormone elements. Genes of the three families were differentially expressed in roots, stems, inflorescences and seeds. HvPYL2 had the highest expression in root; HvPYL9 had the highest expression in episperm; HvPP2C-A1-10 had the highest expression in inflorescence. HvSnRK2.5, HvSnRK2.7, and HvSnRK2.8 had the highest expression in episperm. Transcriptome data analysis of a pair of near-isogenic lines with significant differences in salt tolerance at 48 h and 60 h under salt stress showed that HvPP2C-A2, HvPP2C-A4, HvPP2C-A6, HvPP2C-A9, HvPP2C-A10, and HvSnRK2.4 were significantly up-regulated by salt stress, while HvPYL6 and HvPYL7 were significantly down-regulated. The results of qPCR were consistent with the transcriptome data. The results showed that the PYL, PP2CA, and SnRK2 family played an important role in the response of barley to salt stress. |
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