| 王 丹，郭雅洁，刘梦甜，韩雪莹，李慧芳，姬 祥，刘会云.过表达AtHPT和AtTC对小麦籽粒生育酚含量和产量性状的影响[J].麦类作物学报,2025,(6):727 |
| 过表达AtHPT和AtTC对小麦籽粒生育酚含量和产量性状的影响 |
| Effects of Overexpression of AtHPT and AtTC Genes on Tocopherol Content and Yield Traits of Wheat Grain |
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| DOI： |
| 中文关键词: 小麦 拟南芥 生育酚环化酶 尿黑酸植基转移酶 维生素E |
| 英文关键词:Wheat Arabidopsis thaliana Tocopherol cyclase Homogentisate phytyl transferase Vitamin E |
| 基金项目:河南省自然科学基金项目（232300420189）；河南省科技攻关项目（242102111117）；河南农业大学大学生创新训练计划项目（2022DC008）;国家重点研发计划（2021YFF1000203） |
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| 中文摘要: |
| 小麦作为主要的粮食作物之一，是人类膳食营养的主要来源，通过生物育种手段可以提高小麦籽粒中维生素E特别是α-生育酚的含量。本研究利用农杆菌介导的遗传转化方法将拟南芥AtHPT和AtTC基因分别转入六倍体小麦Fielder中，通过qRT-PCR选择AtHPT和AtTC基因在籽粒中表达量较高的转基因株系，对其生育酚含量进行HPLC检测，并对其株高、粒长、粒宽、千粒重、小穗数和穗粒数进行考种分析。结果发现，过表达AtTC的转基因小麦株系的籽粒中未检测到β-生育酚，δ-生育酚含量与小麦Fielder相比无明显差异，α-生育酚的含量显著提高；转基因株系后代的穗长、小穗数和穗粒数均显著增加。过表达AtHPT的转基因小麦株系的籽粒中未检测到α-生育酚，β-和δ-生育酚含量与小麦Fielder相比均无明显差异。转基因株系均未检测出γ-生育酚。以上结果表明，过表达AtTC可以显著提高小麦籽粒中α-生育酚的含量，并对产量性状无负面影响。 |
| 英文摘要: |
| Wheat, as one of the main cereal crops, is the primary source of human dietary nutrition. Biological breeding methods can increase the content of vitamin E in wheat grains, especially the α-tocopherol. This study Agrobacterium-mediated transferred AtHPT and AtTC genes into hexaploid wheat Fielder, and selected the lines with high expression of AtHPT and AtTC genes in grains through qRT-PCR experiments. HPLC was performed on their tocopherol content, and the plant height, grain length, grain width, thousand grain weight, spikelet number, and grain per spike were determined. The results showed that transgenic wheat overexpression lines of AtTC gene showed no significant difference in the content of δ-tocopherol compared to Fielder, the content of α-tocopherol was significantly increased in transgenic wheat grains, β-tocopherol was no detected, and the agronomic traits of wheat were significantly increased in spike length, number of spikelets, and number of grains per spike in the offspring lines. While the transgenic wheat lines overexpressing the AtHPT gene showed no significant difference in β- or δ-tocopherols content compared to Fielder, α-tocopherol was no detected. γ-tocopherol was no detected in transgenic wheats of AtHPT and AtTC. The above results indicate that overexpression of AtTC can significumtly increase the content of α-taopheral in wheat grains without apparent negative effects on wheat yield traits. |
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