| 王嘉宁，郭 然，李雨洁，曹雨舟，西尔艾力·依不拉衣木，罗 佳,丁 博，李 明，谢晓东，包曙光.小麦气孔发育相关基因TaMUTE的克隆及功能分析[J].麦类作物学报,2025,(6):719 |
| 小麦气孔发育相关基因TaMUTE的克隆及功能分析 |
| Cloning and Functional Analysis of TaMUTE Gene Related to Stomatal Development in Wheat |
| |
| DOI： |
| 中文关键词: 小麦 气孔 TaMUTE基因 功能分析 |
| 英文关键词:Wheat Stoma TaMUTE gene Functional analysis |
| 基金项目:国家大学生创新创业训练计划项目（202310061069）;天津市教委科研计划项目（2020Kj098）;国家自然科学基金（青年）项目（32301817）;天津市“项目+团队”重点培养专项（XC202060） |
|
| 摘要点击次数: 304 |
| 全文下载次数: 183 |
| 中文摘要: |
| 植物气孔通过控制叶片内部与外部环境的气体交换影响植物的蒸腾作用和光合作用，在植物的生长发育和适应环境变化中起着重要作用。为探究气孔发育相关基因TaMUTE在小麦生长发育中的潜在功能，本研究克隆了普通小麦TaMUTE基因并分析了其序列特征，利用qRT-PCR技术分析了在干旱和脱落酸（ABA）胁迫下TaMUTE基因的表达模式，采用转基因技术获得拟南芥TaMUTE转基因植株并分析了其表型。结果表明，从普通小麦中克隆了3个TaMUTE同源基因，将其分别命名为TaMUTE2A、TaMUTE2B和TaMUTE2D。小麦TaMUTE2A的开放阅读框（ORF）为651 bp，编码216个氨基酸；TaMUTE2B的ORF为717 bp，编码238个氨基酸；TaMUTE2D的ORF为648 bp，编码215个氨基酸。3个蛋白属于bHLH家族，均为疏水、不稳定蛋白，其二级结构主要由α-螺旋、延伸链、β-转角和无规则卷曲组成。系统进化分析表明，小麦TaMUTE与大麦HvMUTE的亲缘关系最近。在ABA处理4 h条件下，TaMUTE基因的表达显著上调；在干旱条件下，TaMUTE基因的表达无显著变化。过表达TaMUTE基因会导致转基因拟南芥植株矮小和发育缓慢，增加其气孔密度。 |
| 英文摘要: |
| Stomata affects transpiration and photosynthesis in plants by controlling the gas exchange between the internal and external environments of leaves. It plays an important role in plant growth and development, and adaptation to environmental changes. To explore the potential function of 〖QX(Y15〗TaMUTE〖QX)〗〖KG-1.5mm〗, a gene related to wheat stomatal development, in plant growth and development, was cloned from the common wheat and analyzed for its sequence characteristics. The expression pattern of TaMUTE gene was analyzed under drought and abscisic acid(ABA) stresses by qRT-PCR. The TaMUTE transgenic plants were obtained by transgenic technology and analyzed for their phenotype. The results showed that three TaMUTE homologs gene were cloned from common wheat, which were named TaMUTE2A, TaMUTE2B and TaMUTE2D. The open reading frame(ORF) of the wheat TaMUTE2A gene is 651 bp, encoding 216 amino acids; the ORF of the TaMUTE2B gene is 717 bp, encoding 238 amino acids; and the ORF of the TaMUTE2D gene is 648 bp, encoding 215 amino acids. Three MUTE proteins belong to the bHLH(basic helix-loop-helix) family, which are all hydrophobic and labile proteins. Their secondary structure is mainly composed of α-helix, extended chain, β-turn and irregular recoil. Phylogenetic analysis revealed that TaMUTE is more closely related to the barley MUTE. The expression of the TaMUTE was significantly enhanced after 4 h under ABA treatment. While the expression of the TaMUTE gene did not change significantly under the drought treatment conditions. Overexpression of TaMUTE genes resulted in short stature, development retardation and increased stomatal density in transgenic plant. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
基本信息
