欢迎访问《麦类作物学报》编辑部网站

作者	单位
王子傲，崔永梅，陈玉霞，白羿雄，安立昆，吴昆仑	（1.青海大学农林科学院，青海西宁 810016； 2.省部共建三江源生态与高原农牧业国家重点实验室，青海西宁 810016；3.青海省青稞遗传育种重点实验室，青海西宁 810016； 4.国家麦类改良中心青海青稞分中心，青海西宁 810016； 5.青海大学农牧学院，青海西宁 810016）

摘要点击次数: 326

全文下载次数: 155

中文摘要:

为了研究JA信号途径对青稞苗期耐低温能力的调控作用，以青稞‘昆仑15’为实验材料，对低温胁迫下经过贝他定(Bestatin)和茉莉酸甲酯(MeJA)处理的青稞农艺性状和生理指标进行测定，并对叶和根中JA合成和调控相关基因的表达进行了研究。结果表明，低温处理后青稞株高、根长、植株干(鲜)重、根干(鲜)重、叶绿素(SPAD值)有明显的降低，说明低温胁迫对青稞生长产生了明显的抑制作用。低温胁迫下，Bestatin或MeJA处理明显促进了青稞叶和根中可溶性糖和游离脯氨酸的积累，增强了抗氧化酶(SOD、POD、CAT)活性，并且强烈诱导青稞JA合成和信号途径以及受JA调控的耐低温相关基因表达，说明Bestatin或MeJA处理可以缓解低温胁迫对青稞的伤害，提高青稞对低温的抗性。综合来看，Bestatin和MeJA处理对青稞农艺性状、生理指标及诱导相关基因表达调控的模式一致。

英文摘要:

To investigate the regulatory effect of the jasmonic acid(JA) signaling pathway on low-temperature tolerance in hulless barley seedlings, this study utilized ‘Kunlun 15’ as the experimental material. The agronomic traits and physiological indicators were measured under low-temperature stress treated with Bestatin and methyl jasmonate(MeJA), and the expression levels of JA synthesis and regulation-associated genes in leaves and roots were examined. The result indicated that low-temperature stress substantially decreased plant height, root length, plant dry(fresh) weight, root dry(fresh) weight, and SPAD value in hulless barley, indicating a marked inhibitory effect on growth. Treatment with either Bestatin or MeJA under low-temperature stress notably enhanced the accumulation of soluble sugars and free proline in both leaves and roots, increased the activity of antioxidant enzymes(SOD, POD, CAT), and significantly induced the gene expression of JA synthesis and signaling pathways as well as low temperature tolerance-related genes regulated by JA in hulless barley. These responses suggest that Bestatin and MeJA treatments mitigate low-temperature stress damage in hulless barley by improving its low-temperature resistance. The study elucidated consistent regulatory patterns in agronomic traits, physiological indicators, and gene expression induced by Bestatin and MeJA treatments in hulless barley.

查看全文查看/发表评论下载PDF阅读器

关闭