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作者	单位
官秀娇，杨晓梦，赵塔，杨丽娥，普晓英，李霞，杨砚斌，杨加珍，曾亚文	（1.云南大学资源植物研究院, 云南昆明 650504 2.云南省农业科学院生物技术与种质资源研究所, 云南昆明 650205）

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中文摘要:

为探究不同种植季节及试点对大麦苗粉抗氧化酶活性的影响，以48个云南大麦品种连续两年在滇源镇春、夏、秋、冬四个不同季节播种及滇源、小街、研和镇3个不同地点播种，采用酶标仪测定大麦苗粉超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、多酚氧化酶（PPO）的活性并进行差异分析。结果表明，不同季节和不同地点播种的大麦抗氧化酶活性差异明显，夏播大麦苗粉SOD和 CAT活性显著大于春播、秋播和冬播，而夏播PPO活性略小于秋播，但大于春播与冬播。不同地点播种的大麦苗粉SOD和CAT活性均表现为低海拔的研和镇最高，高海拔的滇源镇最低；PPO活性则表现为小街镇最高，研和镇最低。大麦苗粉抗氧化酶活性主要受播种季节的影响。结合隶属函数法和聚类分析对大麦抗氧化性进行评价，初步筛选出抗氧化酶活性强的大麦优良品种（云啤22号、矮思500、云稞1号、云饲麦7号）。

英文摘要:

To explore the effects of sowing season and site on the antioxidant enzyme activity of barley, 48 barley varieties from Yunnan were sown continuously for two years in Dianyuan town during the four different seasons of spring, summer, autumn, and winter, and also sown at three different altitudes in Dianyuan, Xiaojie, and Yanhe town. The antioxidant enzyme activities of superoxide dismutase(SOD), catalase(CAT), and polyphenol oxidase(PPO) in barley grass powder were determined by microplate reader and differential analysis was conducted. The results showed that there were significant differences in the antioxidant enzyme activity of barley planted in different seasons and at different sites. Under the conditions of four-season planting, the SOD and CAT activities of summer-planted barley grass powder were significantly higher than those of spring, autumn, and winter planting, while the PPO activity of summer planting was slightly lower than that of autumn planting, but higher than that of spring and winter planting. The SOD and CAT activities of barley grass powder at different sites were all highest at the low altitude of Yanhe town but lowest at the high altitude of Dianyuan town. The PPO activity was highest at Xiaojie town but lowest at Yanhe town. The antioxidant enzyme activity of barley grass powder was mainly affected by the planting season. Combining the membership function method and cluster analysis, the antioxidant activity of barley was evaluated, and high-quality barley varieties with high antioxidant enzyme activities were preliminarily screened(Yunpi 22, Aisi 500, Yunke 1, Yunsimai 7).

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