| 王子强，张思娅，张 娜，金开拓，高国庆，吴崇宁，田纪春，邓志英.小麦育种高代品系耐盐优质性的鉴定分析[J].麦类作物学报,2025,(5):563 |
| 小麦育种高代品系耐盐优质性的鉴定分析 |
| Identification and Analysis of Salt-Tolerant and High-Quality Lines in Advanced Wheat Breeding Lines |
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| DOI： |
| 中文关键词: 小麦育种高代品系 耐盐性鉴定 分子标记检测 品质分析 |
| 英文关键词:Wheat elite lines Salt tolerance identification Molecular marker detection Quality analysis |
| 基金项目:山东省自然科学基金项目(ZR2023MC189);山东省重点研发计划(农业良种工程)项目(2023LZGC009-4-2，2022LZGCQY002-3);国家农业重大科技项目(NK20220601)；山东省一流学科建设“811”项目 |
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| 中文摘要: |
| 培育优质耐盐小麦新品种是中国农业发展的一大趋势。本研究以德抗961为对照，对156份小麦育种品系和3份亲本材料，在苗期利用水培法进行不同浓度（0、75和150 mmol·L-1）NaCl盐胁迫处理，分析不同浓度盐胁迫下小麦苗期形态和生理指标，同时，利用优质特异分子标记进行辅助筛选，结合品质性状检测分析，以期获得苗期耐盐性强且品质性状佳的小麦新品系。结果表明，通过对耐盐性综合评价（D值）进行聚类分析，筛选出高耐盐材料7份，耐盐材料42份，中度耐盐材料51份，盐敏感材料24份和高盐敏感材料35份；优质分子标记鉴定发现，Sec-1位点缺失且含有5+10亚基的材料有109份，Sec-1位点与Gli-D2位点均缺失的材料有5份，Gli-D2位点缺失且含有5+10亚基的材料有3份，Sec-1位点和Gli-D2位点同时缺失且含有5+10亚基的小麦材料有3份。综合分析筛选出高耐盐且3个优质基因位点聚合的品系1份，高耐盐且2个优质基因位点聚合的品系3份，高耐盐且Sec-1位点缺失的品系3份。 |
| 英文摘要: |
| The development of high-quality salt-tolerant wheat varieties represents a significant trend in the advancement of agricultural development in China. In this study, Dekang 961 was used as the control, and 156 high-generation wheat breeding lines and three parental materials were subjected to different concentrations(0, 75, and 150 mmol·L-1) of NaCl stress treatments using the hydroponic method during the seedling stage. Meanwhile, high-quality specific molecular markers were used for assisted selection, combined with quality trait detection and analysis, to obtain wheat lines with strong salt tolerance at the seedling stage and excellent quality traits. The results showed that seven highly salt-tolerant materials, 42 salt-tolerant materials, 51 moderately salt-tolerant materials, 24 salt-sensitive materials, and 35 highly salt-sensitive materials were screened through cluster analysis of the comprehensive evaluation value D. Quality molecular marker identification revealed that 109 materials lack Sec-1 locus but possess the 5+10 subunit; five materials lack both the Sec-1 and Gli-D2 loci; three materials lack the Gli-D2 locus but possess the 5+10 subunit, and three materials lack both the Sec-1 and Gli-D2 loci while possess the 5+10 subunit. Comprehensive analysis led to the selection of one line with high salt tolerance and the pyramiding of three high-quality gene loci, three lines with high salt tolerance and the pyramiding of two high-quality gene loci, and three lines with high salt tolerance and the absence of the Sec-1 locus. |
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