| 江 航，马立国，祁 凯，张悦丽，张 博，马国苹，曾 凤，齐军山.禾谷镰刀菌NuA4组蛋白乙酰转移酶复合体亚基FgEaf7的功能研究[J].麦类作物学报,2025,(4):536 |
| 禾谷镰刀菌NuA4组蛋白乙酰转移酶复合体亚基FgEaf7的功能研究 |
| Functional Study of the NuA4 Histone Acetyltransferase Complex Subunit FgEaf7 in Fusarium graminearum |
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| DOI： |
| 中文关键词: 小麦赤霉病 禾谷镰刀菌 NuA4复合体 基因表达 |
| 英文关键词:Fusarium head blight F.graminearum NuA4 complex Gene expression |
| 基金项目:国家自然科学基金项目(32102181)；山东省自然科学基金项目(ZR2021QC059)；山东省现代农业产业技术体系小麦创新团队项目(SDAIT-01-10) |
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| 中文摘要: |
| 由禾谷镰刀菌引起的小麦赤霉病和茎基腐病在中国发生严重，威胁着国家的粮食安全。NuA4组蛋白乙酰转移酶复合体可调控真菌的多种发育过程，但在植物病原真菌中，有关NuA4中多个亚基的功能尚不清楚。为明确NuA4中亚基Eaf7在植物病原真菌中的功能，本研究以禾谷镰刀菌为研究对象，敲除基因FgEAF7，发现Fgeaf7突变体的生长速率显著低于野生型菌株，但基因FgEAF7的缺失不影响分生孢子的产生、对小麦的致病力以及DON毒素的生物合成。Fgeaf7突变体对刚果红和SDS的敏感性下降，表明基因FgEAF7的缺失影响细胞壁的发育。对FgEaf7的结构域进行分析，发现FgEaf7中含有一个保守的Eaf7结构域，将Eaf7结构域敲除后，发现FgEAF7△Eaf7突变体和Fgeaf7突变体的缺陷一致，表明Eaf7结构域对于FgEaf7发挥完整功能是必需的。转录组分析显示，与野生型菌株相比，Fgeaf7突变体中有1 800多个基因差异表达，其中，多个对生长发育有重要作用的基因在Fgeaf7突变体中下调表达。推测FgEaf7主要调控禾谷镰刀菌营养生长和细胞壁的发育等过程。 |
| 英文摘要: |
| Fusarium head blight and Fusarium crown rot caused by Fusarium graminearum occurs severely in China, posing a threat to national food security. The NuA4 histone acetyltransferase complex regulates various developmental processes in fungi, but the functions of multiple subunits within the NuA4 complex in plant pathogenic fungi are currently unclear. To elucidate the function of Eaf7 subunit in plant pathogenic fungi, this study used F. graminearum as a model organism. Through knocking out the FgEAF7 gene, it was found that the growth rate of the Fgeaf7 mutant was significantly lower than that of the wild-type strain. However, the deletion of FgEAF7 gene did not affect conidiation, pathogenicity to wheat, and DON biosynthesis. Additionally, we found that the Fgeaf7 mutant showed decreased sensitivity to Congo red and SDS, indicating that the deletion of FgEAF7 gene affects cell wall development. Analysis of the FgEaf7 domain revealed a conserved Eaf7 domain within FgEaf7. Upon deletion of the Eaf7 domain, the FgEAF7△Eaf7 mutant exhibited similar defects to the Fgeaf7 mutant, suggesting that the Eaf7 domain is essential for the full function of FgEaf7. Transcriptomic analysis showed that over 1 800 genes were differentially expressed in the Fgeaf7 mutant, compared to the wild type, with several genes important for growth and development being downregulated. These findings indicate that FgEaf7 regulates various processes, such as vegetative growth and cell wall development in F.graminearum. |
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