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郭 阳,卢恩钰,周小妹,郭青云,康振生,姚 强.208份春小麦资源抗条锈性评价及抗病基因检测[J].麦类作物学报,2025,(4):448
208份春小麦资源抗条锈性评价及抗病基因检测
Evaluation of Stripe Rust Resistance and Detection of Disease Resistance Genes in 208 Spring Wheat Resources
  
DOI:
中文关键词:  小麦条锈病  抗病性评价  抗条锈病基因  分子检测  基因芯片
英文关键词:Wheat stripe rust  Evaluation of disease resistance  Stripe rust resistance gene  Molecular detection  Gene array
基金项目:国家重点研发计划项目(2021YFD1401000);青海省重点研发与转化计划项目(2022-NK-125)
作者单位
郭 阳,卢恩钰,周小妹,郭青云,康振生,姚 强 (1.青海大学农林科学院/青海省农业有害生物综合治理重点实验室青海西宁 810016 2.西北农林科技大学作物抗逆与高效生产全国重点实验室,陕西杨凌 712100) 
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中文摘要:
      青海是中国小麦条锈病的越夏菌源区之一。为探明青海现有小麦生产品种和后备种质资源对小麦条锈病的抗性水平及抗条锈病基因的分布,本研究利用小麦条锈菌生理小种CYR31和优势生理小种CYR32、CYR33、CYR34对208份供试小麦资源进行苗期和成株期抗病性鉴定,并利用基因芯片检测其所携带的抗条锈基因。结果表明,80份(38.5%)材料对CYR31、CYR32、CYR33和CYR34均表现抗病;结合成株期抗病鉴定结果,125份(60.1%)材料表现成株抗条锈性,74份(35.6%)材料表现为全生育期抗性。通过分子检测, 204份(98.1%)、114份(54.8%)、70份(33.7%)、66份(31.7%)、59份(28.4%)、54份(26.0%)、45份(21.6%)、31份(14.9%)、16份(7.7%)、1份(0.5%)、193份(92.8%)、168份(80.8%)、147份(70.7%)、124份(59.6%)、107份(51.4%)、86份(41.3%)、77份(37.0%)、36份(17.3%)、26份(12.5%)、20份(9.6%)、18份(8.7%)材料分别携带Yr29Yr78YrZH58Yr5Yr75Yr80Yr30Yr26Yr82YrSPQYrqin-2ALQYr.nwafu-3BSQYrsn.nwafu-1BLQYrqin-6BSQYrxn-1BLQYrqin-2BLQYrsn-6BSQYrsn-2ASQYrsn-3DLQYrhm-2BCQYr-4BL基因。本研究检测了当前青海省小麦种质资源抗病性水平及所携带的抗病基因,可为小麦抗病育种及抗病基因的合理布局提供理论依据。
英文摘要:
      Qinghai Province is one of Chinas over-summering pathogen source areas of wheat stripe rust. To explore the resistance level and the distribution frequency of stripe rust resistance genes of the wheat varieties and germplasm resources in Qinghai Province, the resistance of 208 wheat resources at seedling and adult stages was identified using the physiological race CYR31 and dominant physiological races CYR32, CYR33, CYR34 of wheat stripe rust. The stripe rust resistance genes were detected by gene array. The results showed that 80 wheat varieties(accounting for 38.5% in total) were resistant to CYR31, CYR32, CYR33, and CYR34. Combined with the results of disease resistance identification at the adult stage, 125(accounting for 60.1% in total) materials showed adult plant resistance to stripe rust, 74(accounting for 35.6% in total) materials showed resistance during the whole growth period. The results of molecular detection showed that 204(98.1%), 114(54.8%), 70(33.7%), 66(31.7%), 59(28.4%), 54(26.0%), 45(21.6%), 31(14.9%), 16(7.7%), 1(0.5%), 193(92.8%), 168(80.8%), 147(70.7%), 124(59.6%), 107(51.4%), 86(41.3%), 77(37.0%), 36(17.3%), 26(12.5%), 20(9.6%), and 18(8.7%) materials carried Yr29, Yr78,YrZH58,Yr5, Yr75, Yr80, Yr30, Yr30, Yr26,Yr82, YrSP, QYrqin-2AL,QYr.nwafu-3BS, QYrsn.nwafu-1BL, QYrqin-6BS, QYrxn-1BL, QYrqin-2BL, QYrsn-6BS, QYrsn-2AS, QYrsn-3DL, QYrhm-2BC and QYr-4BL, respectively. This study systematically revealed the current level of disease resistance and the resistance genes carried by wheat germplasm resources in Qinghai Province. It provides a theoretical basis for wheat disease resistance breeding and the rational distribution of disease resistance genes.
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