| 毕珍珍，郑 艳，李 辉，李晓建，刘 雯，母学荣，朱 娜，郑佳乐，宋瑜龙，闵东红，张小红.小麦DH系创制中培养基、秋水仙浓度的筛选及其HMW-GS组成分析[J].麦类作物学报,2025,(4):432 |
| 小麦DH系创制中培养基、秋水仙浓度的筛选及其HMW-GS组成分析 |
| Screening of Culture Medium and Concentration of Colchicine for Construction of Wheat DH Lines and Their HMW-GS Composition Analysis |
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| DOI： |
| 中文关键词: 小麦 花药培养 DH系 HMW-GS |
| 英文关键词:Wheat Anther culture Double haploid HMW-GS |
| 基金项目:科技创新2030-重大项目（2023ZD0402601） |
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| 摘要点击次数: 347 |
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| 中文摘要: |
| 为了提高小麦DH系创制效率，以11个小麦杂交组合的F1代为材料，比较分析了两种培养基（NPB-99和CHB）下小麦花药的诱导效率以及三种浓度秋水仙素处理（4、6和8 g·L-1）下单倍体的染色体加倍效率，并利用SDS-PAGE对获得的DH株系进行优质亚基检测。结果表明，CHB培养基花药诱导效率优于NPB-99培养基。在三种秋水仙素浓度中,6 g·L-1诱导单倍体加倍的效率最高。在所获得的33个DH系中共鉴定出16个株系携带优质亚基组合（1/7+8/5+10或1/7+9/5+10），且具有较好的烘焙品质，可作为小麦品质育种的种质资源。 |
| 英文摘要: |
| In order to improve the construction of wheat DH lines, the F1 generation of 11 wheat hybrid combinations was used as materials to compare and analyze the induction efficiency of wheat anthers under two media(NPB-99 and CHB) and the chromosome doubling efficiency of haploids under three concentrations of colchicine treatments(4, 6 and 8 g·L-1). The high-molecular-weight glutenin subunits(HMW-GS) of the obtained DH lines were identified by SDS-PAGE. The results showed that the anther induction efficiency of CHB medium was better than that of NPB-99 medium. The concentration of 6 g·L-1 colchicine was more efficient in inducing haploid doubling. A total of 16 lines carrying prior HMW-GS combinations(1/7+8/5+10 or 1/7+9/5+10) were identified from the 33 DH lines, with good baking quality, which could provide genetic resources for subsequent wheat quality breeding research. |
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