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为了挖掘更多与小麦株高相关且适合标记辅助育种需求的QTL，采用长江中下游麦区骨干亲本扬麦158和西风配制重组自交系群体，使用55K芯片技术分析群体基因型并构建遗传连锁图，联合4个年度群体株高表型资料，对影响群体株高的QTL进行分子定位。结果表明，群体遗传连锁图含有3 830个SNP标记，覆盖2 784.9 cM，标记间平均距离0.7 cM；21条染色体共定位了39个可重复的与株高相关QTL，其中18个QTL为2个年度可重复定位，8个QTL为3个年度可重复定位，13个QTL为4个年度可重复定位，单个QTL可解释3.2%~13.6%的株高表型变异。4个年度可重复定位的QTL分别来自西风的1B、1D、4A、4B、4D、5A、5B、7A染色体以及扬麦158的3A、3D、6A、7B、7D染色体，其中扬麦158贡献的QPh·jaas-3A.3和QPh·jaas-7B.1分别解释8.4%~13.4%以及9.1%~13.1%的株高变异。这些株高QTL位点有望为长江中下游麦区小麦分子标记辅助育种提供帮助。

英文摘要:

Wheat plant height is an important agronomic trait affecting wheat yield, harvest index and lodging resistance of the plants, and cultivating wheat with proper plant height for production needs is one of the important goals of breeders. In order to explore more QTLs related to wheat plant height and suitable for marker-assisted breeding, the recombinant inbred line population was developed by Yangmai 158 and Xifeng, the backbone parents of the wheat area in the middle and lower reaches of the Yangtze River. The population genotype was analyzed by 55K SNP array and the genetic linkage map was constructed by JoinMap software. Combining the phenotypic data of plant height in four years, the QTLs associated with wheat plant height were mapped in the chromosomes. The result showed that the genetic linkage map containing 3 830 SNPs distributed in 2 784.9 cM of the wheat genome and the average distance between markers was 0.7 cM. A total of 39 repeatable plant height-related QTLs were detected on 21 chromosomes of wheat, of which 18 QTLs were only revealed in 2 years; 8 QTLs were found in 3-year data analysis, and 13 QTLs were mapped in 4 years. A single QTL explained 3.2%-13.6% of the plant height phenotypic variation. The stable QTLs mapped in the four years were individually derived from chromosomes 1B, 1D, 4A, 4B, 4D, 5A, 5B, and 7A of Xifeng and chromosomes 3A, 3D, 6A, 7B, and 7D of Yangmai 158. The QPh·jaas-3A.3 andQPh·jaas-7B.1 contributed by Yangmai 158 could explain 8.4%-13.4% and 9.1%-13.1% of the plant height phenotypic variation, which are helpful for molecular marker-assisted breeding of wheat in the middle and lower reaches of the Yangtze River.

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