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| 小麦TaNF-YB基因家族的鉴定及TaNF-YB-2基因的表达 |
| Identification of the TaNF-YB Gene Family and Expression of TaNF-YB-2 Gene in Wheat |
| 投稿时间：2025-03-27 修订日期：2025-12-08 |
| DOI： |
| 中文关键词: 小麦 NF-YB基因家族 胁迫处理 表达分析 |
| 英文关键词:TriticumSaestivum NF-YB gene family Stress Treatment Expression Analysis |
| 基金项目:联合科研基金一般项目(24JRRA840)；甘肃省科技计划资助(24CXNA038)；国家现代农业产业技术体系项目(CARS-05-04B-2)；甘肃省教育厅产业支撑计划项目(2021CYZC-12)；甘肃省科技计划资助(24CXNA028)；甘肃省自然科学基金(21JR7RA801;20JR10RA507)；国家自然科学(31860377)；甘肃农业大学伏羲青年英才计划(Ganfx-03Y06GAUfx-04Y011)；甘肃省科技支撑计划项目(1604NKCA052-2)；2023 年甘肃省级重点人才项目、甘肃省农业科技支撑项目(KJZC-2023-2)；甘肃省优秀研究生“创新之星”项目(2022CXZXB-028)；甘肃省科技重大专项(17ZD2NA016)第一作者E-mail:1685272603@qq.com(雷蕊伶)通讯作者E-mail:libc@gsau.edu.cn(李葆春) |
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| 中文摘要: |
| NF-YB作为一类关键的转录因子，在植物正常生长发育和逆境胁迫应答中发挥调控作用。本研究利用Hmmer和Blast鉴定小麦NF-YB家族成员，并对其进行理化性质、系统进化及基因结构等生物信息分析，以兰天134、陇紫麦1号和长6878等小麦为研究材料，分析了苗期TaNF-YB-2基因在干旱胁迫和盐胁迫下的表达模式。结果表明：小麦全基因组中共鉴定出10个TaNF-YB家族成员，依次命名为TaNF-YB-1~TaNF-YB-10，该家族成员均为不稳定的酸性蛋白。普通小麦中的TaNF-YB家族成员与斯卑尔脱小麦中的NF-YB基因家族成员具有较高的相似性，属于进化保守型。TaNF-YBs基因主要位于第一和第四同源群，且存在5组基因呈现共线性特征。顺式作用元件分析显示TaNF-YB基因家族与胁迫响应有关。本实验中干旱和盐胁迫处理后，TaNF-YB-2基因在小麦根系和叶片中表达量均呈上升趋势；干旱处理下，3份抗旱小麦材料（兰天134、陇紫麦1号和长6878）中TaNF-YB-2基因的相对表达量始终显著高于2份干旱敏感型材料（西科麦518和兰航选121）；盐胁迫下处理4 h后的叶片和处理6 h后的根系中，3份耐盐小麦材料（兰天134、陇紫麦1号和长6878）中TaNF-YB-2基因的相对表达量均持续显著高于2份盐敏感型材料（西科麦518和兰航选121）。 |
| 英文摘要: |
| NF-YB transcription factors play key regulatory roles in plant growth, development, and stress responses. In this study, HMMER and BLAST were used to identify members of the wheat NF-YB gene family, and their physicochemical properties, phylogenetic relationships, and gene structures were analyzed. Using wheat varieties including Lantian 134, Longzimai 1, and Chang 6878, the expression pattern of TaNF-YB-2 at the seedling stage under drought and salt stress was examined. A total of 10 TaNF-YB family members were identified in the wheat genome and sequentially named TaNF-YB-1 to TaNF-YB-10. These proteins were unstable and acidic. The TaNF-YB members in common wheat showed high similarity to their homologs in spelt wheat, indicating evolutionary conservation. TaNF-YBs were mainly located on homologous groups 1 and 4, with five gene pairs showing collinearity. Cis-element analysis suggested that TaNF-YB genes are involved in stress responses. Under drought and salt stress, the expression of TaNF-YB-2 increased in both roots and leaves. Under drought stress, the relative expression levels of TaNF-YB-2 in three drought-resistant varieties (Lantian 134, Longzimai 1, and Chang 6878) were consistently significantly higher than in two drought-sensitive varieties (Xikemai 518 and Lanhangxuan 121). Under salt stress, the relative expression levels of TaNF-YB-2 in the three salt-tolerant varieties were significantly higher than in the salt-sensitive materials—in leaves after 4 h and in roots after 6 h of treatment. |
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