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作者	单位
贺钟锐，徐浩，闫伟宁，王金硕，王新锐，周子杰，张培培，李在峰	（河北农业大学植物保护学院，河北保定 071000）

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中文摘要:

小麦抗叶锈基因Lr3ka是一个苗期抗病基因，对中国多数叶锈菌生理小种有较高水平抗性。为了解更多Lr3ka基因信息，本研究利用叶锈菌生理小种FHJR对小麦RL6007（携带Lr3ka）、Thatcher及其后代F₂群体进行苗期抗叶锈病鉴定，结合遗传分析及分子标记对Lr3ka进行染色体定位。结果表明，在苗期，RL6007表现抗病，Thatcher表现感病，其后代F₂群体出现分离，卡方检测抗感单株个数符合1∶3的分离比例(??²=1.362，P=0.243>0.05)，推测Lr3ka是一个隐性主效抗叶锈基因。基于中国春参考基因组（IWGSC v2.1）开发了100个SSR标记，利用抗感亲本间的多态性筛选到5个标记，分别为ZBSF6BL-5、ZBSF6BL-12、ZBSF6BL-61、ZBSF6BL-99、ZBSF6BL-100；利用5个分子标记检测F₂群体，将Lr3ka基因定位于6BL染色体上，位于ZBSF6BL-61和ZBSF6BL-99之间，遗传距离均为0.1 cM；对应中国春参考基因组序列v2.1物理区间716.2~730.7 Mb。开发的SSR分子标记可以用于该基因的分子标记辅助选择， Lr3ka的定位可为其进一步利用提供参考。

英文摘要:

The known wheat leaf resistance gene Lr3ka is a seedling resistance gene exhibiting high levels of resistance in China with important application potentiality. In this study, to know more information of Lr3ka, leaf rust pathotype FHJR was used to inoculate RL6007(carrying Lr3ka), Thatcher and F₂ populations to identify the leaf rust resistance at seedling stage. Genetic analysis and molecular markers were used to map Lr3ka. Results showed that RL6007 was resistant, while Thatcher was susceptible. The F₂ population showed segregation, and chi-square tests confirmed the expected 1∶3 ratio(χ²=1.362, P=0.243>0.05), indicating Lr3ka is a recessive major resistance gene. A total of 100 SSR markers were developed according to the wheat reference sequence of Chinese Spring(IWGSCv2.1), and five SSR markers,namely ZBSF6BL-5,ZBSF6BL-12,ZBSF6BL-61,ZBSF6BL-99,and ZBSF6BL-100, showed good polymorphism between parents. The five markers were used to detect the population, Lr3ka was mapped on 6BL, flanking by SSR marker ZBSF6BL-61 and ZBSF6BL-99 both with genetic distance of 0.1 cM. The physical interval of Lr3ka is 716.2-730.7 Mb corresponding to Chinese Spring reference genome sequence V2.1. The SSR markers can be used for marker-assisted selection(MAS), and it provided reference for the utilization of the gene.

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