| 谢 妍，吴林楠，张天星，李 梦，马 猛.小麦株型相关基因TaOTUB1的克隆和功能标记开发[J].麦类作物学报,2025,(3):279 |
| 小麦株型相关基因TaOTUB1的克隆和功能标记开发 |
| Cloning and Functional Marker Development of Wheat Plant Type-Related Gene TaOTUB1 |
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| DOI： |
| 中文关键词: 小麦 株型 TaOTUB1 功能标记 |
| 英文关键词:Wheat Plant type TaOTUB1 Functional marker |
| 基金项目:国家自然科学基金项目(32072059) |
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| 中文摘要: |
| 为了开发小麦株型相关的功能标记,对小麦OTUBs家族成员进行鉴定,并克隆得到一个潜在株型相关基因TaOTUB1，并对其表达模式进行分析。结果表明，TaOTUB1基因在小麦各组织广泛表达，且在小麦OTUBs家族33个成员中表达丰度最高。利用748份小麦基因组自然变异数据分析发现，TaOTUB1-A启动子区主要存在HapⅠ和HapⅡ两种单倍型。基于-761 bp位点的SNP开发dCAPS分子标记，利用该标记能够在313份小麦品种中有效鉴定HapⅠ和HapⅡ。通过关联分析，HapⅠ是有利于小麦株型改良和产量增加的优异单倍型。HapⅠ在国内外栽培小麦中属于优势单倍型。研究结果有助于小麦株型改良和分子标记辅助选择育种。 |
| 英文摘要: |
| To develop functional markers related to wheat plant type, we identified members of the wheat OTUBs family in this study, cloned a potential plant type-related gene, TaOTUB1, and analysed the expression pattern of the gene. The result showed that TaOTUB1 gene was widely expressed in various tissues of wheat and had the highest expression abundance among the 33 members of the wheat OTUBs family. The natural variation of the TaOTUB1 gene was analyzed using 748 wheat genome natural variation data, and it was found that two haplotypes, HapⅠ and HapⅡ, mainly existed in the TaOTUB1-A promoter region. The development of a dCAPS molecular marker based on SNPs at the -761 bp locus enabled the effective identification of HapⅠ and HapⅡ in 313 wheat varieties. Linkage analysis revealed that HapⅠ is an excellent haplotype for plant type improvement and yield increase. Evolutionary analysis revealed that HapⅠ is the dominant haplotype in domestic and foreign-cultivated wheat. These results can be helpful for plant type improvement and molecular marker-assisted selection breeding in wheat. |
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