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作者	单位
邓云颢，李鲁华，徐灵，王忠妮，洪鼎立，邰成江，徐如宏	（1.贵州大学农学院，贵州贵阳 550025；2.国家小麦改良中心贵州分中心，贵州贵阳 550025；3.贵州省农业科学院水稻研究所，贵州贵阳 550025）

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中文摘要:

PBF因子是小麦胚乳特异基因表达调控蛋白因子之一，在调控小麦种子贮藏蛋白基因表达方面有着重要作用。本研究以小麦种质ZY96-3为材料，利用RT-PCR技术克隆获得TaPBF3-5D的CDS序列，对其进行了生物信息学和基因表达分析。结果表明，TaPBF3-5D基因CDS序列共有909 bp，编码302个氨基酸；其编码的蛋白为一个非跨膜的不稳定性亲水蛋白，无信号肽，共含36个磷酸位点；预测其定位于细胞核；通过系统进化树以及多重序列比对，TaPBF3-5D与来自于普通小麦中国春的PBF在亲缘性上更为接近。经荧光定量分析，TaPBF3-5D基因在根、茎、叶和籽粒中均有表达，以籽粒中表达最高，其次是叶和茎，根中表达最低。这进一步说明TaPBF3-5D基因参与调控小麦籽粒贮藏蛋白基因表达，调节籽粒发育。

英文摘要:

PBF factor is one of the protein factors regulating the expression of wheat endosperm specific gene, and plays an important role in regulating the expression of wheat seed storage protein genes. In this study, the CDS sequence of TaPBF3-5D was obtained from wheat germplasm ZY96-3 by RT-PCR, and its bioinformatics and gene expression were analyzed. The results showed that the CDS sequence of TaPBF3-5D had a total of 909 bp, encoding 302 amino acids. The encoded protein is a non-transmembrane unstable hydrophilic protein without signal peptide, and it contains 36 phosphoric acid sites. It is predicted to be located in the nucleus. Phylogenetic tree and multiple sequence alignment showed that TaPBF3-5D was more closely related to the PBF from common wheat. Quantitative fluorescence analysis showed that TaPBF3-5D gene was expressed in roots, stems, leaves, and seeds, with the highest expression in seeds, followed by leaves and stems, and the lowest expression in roots. It indicated TaPBF3-5D gene regulating the grain development of wheat through participating in the regulation of wheat grain storage protein gene expression.

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