| Color-grained wheat is rich in anthocyanins, phenols and other bioactive components, representing an important genetic resource for improving wheat nutritional quality. To date, more than 60 color-grained wheat cultivars have been developed and promoted in China, but no information is available on their chromosome diversity. In this study, 50 purple-grained wheat cultivars(lines) collected from different regions of China were analyzed for chromosome constitutions using oligonucleotide probe multiplex#7(ONPM#7) FISH. The results showed that 49 cultivars(lines) were homozygous and 1 cultivar was heterozygous. A total of 50 karyotypes were further developed. A total of 113 chromosome polymorphic types were found, ranging from 2.0% to 100%, and 12 of polymorphic chromosomes showed a frequency more than 50%, representing the dominant types. Chromosome polymorphic information content(CPIC) analysis showed that A subgenome varied the largest, followed by B subgenome, and D subgenome the least. Among single chromosomes, 6A varied the largest, while 3D and 4D the least. Based on the polymorphic chromosomes, 50 cultivars(lines) were grouped into four subgroups: G1-1 and G1-2 respectively containing 4 and 8 accessions, all of which carry T1BL·1RS, and G2-1 and G2-2 containing 12 and 26 accessions, all of which carry non-T1BL·1RS. The results indicated that ONPM#7 was a powerful tool for color-grained wheat chromosome identification. |
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