| Drought is the main environmental stress factor affecting barley production. In order to understand the changes of antioxidant enzyme activity and related gene expression in barley under drought stress, a drought-resistant barley variety ZDM5430 and a drought-sensitive barley variety IL-12 were used as materials. The seedlings were treated by drought for 21 days and then rewatered for 7 days to study the differences of antioxidant enzyme activity and gene expression in leaves of two barley varieties. The results showed that compared with the control, the relative water content(RWC) and chlorophyll(Chl) content of the leaves decreased significantly under drought stress; the contents of malondialdehyde(MDA), superoxide anion radical(O2-·) and hydrogen peroxide(H2O2) increased significantly, and the activities of superoxide dismutase(SOD), peroxidase(POD), ascorbate peroxidase(APX) and catalase(CAT) increased significantly. Compared with the drought resistant variety ZDM5430, the sensitive variety IL-12 showed a greater decrease in RWC and Chl content under drought stress, while the MDA, O2-·, and H2O2 contents increase. There was no significant difference in SOD, POD, APX and CAT activities between the two varieties. Under drought stress, the expression levels of antioxidant genes GPX4, CAT, Cu/Zn SOD, 2-Cys POD, Cyt-APX and DHAR in drought resistant variety ZDM5430 were higher than those in drought sensitive variety IL-12. After rewatering, ZDM5430 and IL-12 showed varying degrees of compensatory effects on various physiological indicators, with an increase in RWC and Chl content and a decrease in MDA content, O2-·content, H2O2content, SOD activity, POD activity, APX activity, and CAT activity. Meanwhile, the expression levels of GPX4, CAT, Cu/Zn SOD, 2-Cys POD, Cyt-APX, and DHAR genes were significantly reduced. |
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