欢迎访问《麦类作物学报》编辑部网站

作者	单位
王梦玥，罗振蒙，詹开旺，毛孝强，周洪斌，魏红霞，高洪铭，陈升位	（1.云南农业大学农学与生物技术学院，云南昆明 650201； 2.沾益区植保植检站，云南曲靖 655331）

摘要点击次数: 498

全文下载次数: 279

中文摘要:

Nop（nucleolar protein）结构域蛋白可参与核糖体装配、核苷酸甲基化修饰、基因表达调控等生物进程，是制约真核生物发育遗传、细胞周期调控和衰老机制等研究的关键因素，但少有大麦Nop结构域核仁蛋白基因的报道。本研究以北青7号和Ynbs突变体为材料，克隆了大麦HvNOP58基因的CDS序列。结果表明，HvNOP58基因位于大麦Chr2H基因组负链的49 019 790~49 023 604 bp区间，具有10个外显子，编码区长度为1 674 bp。所编码蛋白属于亲水性的稳定蛋白，其高级结构以α-螺旋和无规则卷曲为主，具有N端结构域、NOSIC结构域和Nop结构域。同源比对和系统进化分析表明，HvNOP58蛋白与同族物种的核仁蛋白具有更近的亲缘关系。亚细胞定位、RT-PCR检测和荧光定量检测结果表明，HvNOP58基因定位于细胞核，在叶、茎、幼穗、根和叶鞘5种组织中有表达。

英文摘要:

The protein with nucleolar protein(Nop) domain can participate in biological processes, such as ribosome assembly, nucleotide methylation modification and gene expression regulation, and is a key factor that restricts the development and inheritance, cell cycle regulation and aging mechanisms of eukaryotes. However, there were few reports on the nucleolar protein genes with Nop domain in barley. In the present study, the coding sequence of barley gene HvNOP58 was cloned using Beiqing 7 and Ynbs mutant as materials. The results showed that HvNOP58 gene was located within 49 019 790-49 023 604 bp range of the negative strand of the Chr2H in barley, and contained 10 exons, a coding region containing 1 674 bp. The putative protein encoded by HvNOP58 belonged to a hydrophilic stable protein, and its advanced structure is characterized by some α-helices and random coils, containing the N-terminal domain, NOSIC domain and Nop domain. Homology comparison and phylogenetic analysis indicated that the HvNOP58 protein showed a closer phylogenetic relationship with nucleolar proteins of the same species. Based on subcellular localization, RT-PCR detection and fluorescence quantification, the HvNOP58 gene was located in the nucleolus and expressed of in the leaves, stems, young spikes roots, and leaf sheaths.

查看全文查看/发表评论下载PDF阅读器

关闭