欢迎访问《麦类作物学报》编辑部网站

作者	单位
王盛锋，陈磊，江华波，高丽丽，乙引，汪洪	(1.中国农业科学院农业资源与农业区划研究所/农业农村部植物营养与肥料重点实验室,北京 100081；2.新洋丰农业科技股份有限公司/养分资源高效利用湖北省工程研究中心，湖北荆门 448000 3.黑龙江省黑土保护利用研究院, 黑龙江哈尔滨 150086 4.贵州师范大学生命科学院,贵州贵阳 550001)

摘要点击次数: 529

全文下载次数: 314

中文摘要:

为探讨不同硝态氮供应水平下外源钙对小麦根系生长发育的影响，通过水培实验，在不同浓度硝态氮（0、2.5、50.0 mmol·L^-1）下设置0 mmol·L^-1 CaCl₂（缺钙）、2 mmol·L^-1 CaCl₂（正常供钙）和2 mmol·L^-1 CaCl₂+6 mmol·L^-1 EGTA（Ca²⁺螯合剂）三个外源钙梯度，比较分析了不同处理下小麦地上部鲜重及根系长度、表面积等形态指标的差异。结果表明，与2.5 mmol·L^-1硝态氮处理相比，0和50 mmol·L^-1（高浓度）硝态氮供应下，小麦地上部鲜重、侧根长度、根系表面积、根系体积均明显减少，根系生长受到抑制。无硝态氮(0 mmol·L^-1)供应下，缺钙与EGTA施用对小麦根系侧根长度、侧根总数、次生根数、平均侧根长度均无明显影响。在硝态氮供应条件下，缺钙与EGTA施用后小麦根系伸长受抑，初生根长度、侧根长度、根系总长度均显著变短；一、二级侧根数及侧根总数和根系表面积均明显减少；直径>0.45 mm的根系所占比例增加，根系变粗。缺钙和添加EGTA对小麦根系生长发育影响表现一致。综合分析，硝态氮供应水平对小麦根系生长发育具有重要调控作用，外源钙可能参与了硝态氮的这一生物学功能。

英文摘要:

In order to understand the effect of calcium on root growth of wheat under different nitrate levels, a hydroponics experiment was conducted with 0 mmol·L^-1 CaCl₂ (no Ca²⁺), 2 mmol·L^-1 CaCl₂ (normal Ca²⁺supply) and 2 mmol·L^-1 CaCl₂+6 mmol·L^-1 EGTA (a Ca²⁺ chelating agent)under the three nitrate levels of 0, 2.5 and 50.0 mmol·L^-1. The differences of the shoot fresh weight and root morphology indices of wheat among different treatments were analyzed. The results showed that compared with 2.5 mmol·L^-1 of nitrate level, the shoot biomass, lateral root length, root surface area, root volume of wheat were significantly reduced under the nitrate supply of 0 mmol·L^-1 and 50 mmol·L^-1 (high concentration). Compared to the normal calcium treatment, calcium deficiency and EGTA (Glycol bis (a aminoethylether) N,N,N′,N′ tetraacetic acid) application did not affect the length of lateral roots, the total number of lateral roots, root sections, average lateral length of wheat under no nitrate supply, but significantly decreased the lateral root length, primary root length, total root length, the primary and secondary lateral root number, the total number of lateral roots and root surface area, increased the proportion of root with the diameter more than 0.45 mm and root system under nitrate supply. The effects of calcium deficiency and added EGTA treatments on wheat root growth and development were consistent. It is suggested that nitrate level played a regulatory role in wheat root growth, and exogenous calcium may be involved in the nitrate biological function.

查看全文查看/发表评论下载PDF阅读器

关闭