| 黄昱箫，黄思曙，占传松，刘贤青，许从萍，曾兴权，罗 杰.代谢组分析青稞芳香族酚胺响应白粉病胁迫[J].麦类作物学报,2024,(8):1019 |
| 代谢组分析青稞芳香族酚胺响应白粉病胁迫 |
| Metabolome Analysis of the Response of Barley Aromatic Phenolamides to Powdery Mildew Stress |
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| DOI： |
| 中文关键词: 青稞 白粉病 代谢组学 芳香族酚胺 |
| 英文关键词:Qingke Powdery mildew Metabolomics Aromatic phenolamides |
| 基金项目:国家自然科学基金项目（31625021） |
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| 摘要点击次数: 468 |
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| 中文摘要: |
| 大麦专化型白粉菌（Blumeria graminis f. sp. hordei，Bgh）引起的白粉病对青稞的产量和质量造成了严重影响。本研究采用广泛靶向代谢组学技术，对青稞品种G72（白粉病敏感型）和K69（白粉病高抗型）进行了分析，旨在探究青稞抵御白粉病的相关代谢物途径。结果表明：共鉴定出406种代谢物，包括氨基酸衍生物、黄酮类化合物、酚胺、苯丙烷、脂类、有机酸、核苷酸等次生代谢物；主成分分析（PCA）显示，高抗品种和易感品种在白粉菌侵染后的代谢物积累模式存在差异； KEGG富集分析表明，与G72相比， K69在苯丙烷和类黄酮等生物合成途径上代谢物显著富集；在白粉菌接种24 h后，高抗性青稞品种K69和大麦品种WDM00496在类黄酮途径中的代谢物含量均显著提高，而K69的酚胺类化合物，尤其是芳香族酚胺的积累显著增加。外源喷施芳香族酚胺的实验发现，芳香族酚胺的积累可以增强青稞抵御白粉菌侵染的能力。综上所述，芳香族酚胺是青稞抗白粉病的关键代谢物，其在白粉菌的诱导下积累，从而增强了青稞的抗白粉病能力。 |
| 英文摘要: |
| Powdery mildew caused by the specialized fungus Blumeria graminis f. sp. hordei(Bgh) has a severe impact on the yield and quality of hulless barley. In this study, we employed a comprehensive metabolomics approach to analyze two hulless barley varieties, G72(susceptible to powdery mildew) and K69(highly resistant to powdery mildew), with the aim of exploring the metabolic pathways associated with hulless barley resistance to powdery mildew. The results indicated that a total of 406 metabolites were identified, including secondary metabolites such as amino acid derivatives, flavonoids, phenolamides, phenylpropanoids, lipids, organic acids, and nucleotides. Principal component analysis(PCA) revealed distinct metabolic patterns between the resistant and susceptible varieties during Bgh infection. KEGG enrichment analysis showed that, compared to variety G72, variety K69 exhibited a significant enrichment of metabolites in biosynthetic pathways of phenylpropanoids and flavonoids. Upon inoculation with powdery mildew for 24 h, the resistant hulless barley variety K69 and WDM00496 both demonstrated a significant increase in the content of metabolites in the flavonoid pathway, while the variety K69 showed a particularly pronounced accumulation of phenolamides compounds, especially aromatic phenolamides. Exogenous application of aromatic phenolamides was found to enhance the ability of hulless barley to resist Bgh infection. In summary, aromatic phenolamides are key metabolites in hulless barley resistance to powdery mildew, and their accumulation upon induction by powdery mildew enhanced the hulless barley resistance to the disease. |
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